Diversity of Methicillin-resistant and -susceptible in Clinical Strains from Malaysia: a High Prevalence of Invasive European -type T032

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2021 Apr 23

PMID 33889447

Citations 7

Authors

Sherry Usun Jones

Kek Heng Chua

Ching Hoong Chew

Chew Chieng Yeo

Fatimah Haslina Abdullah

Norlela Othman

Boon Pin Kee

Suat Moi Puah

Affiliations

Soon will be listed here.

Abstract

Background: is one of the important pathogens causing nosocomial infection. typing allows identification of clones in hospital isolates and is useful for epidemiological studies and nosocomial infection control. This study aims to investigate the types in Malaysian isolates obtained from various clinical specimens.

Method: A total of 89 methicillin-resistant (MRSA) [pus ( = 55), blood ( = 27), respiratory ( = 5), eye ( = 2)] isolates and 109 methicillin-susceptible (MSSA) [pus ( = 79), blood ( = 24), respiratory ( = 3), eye ( = 2) and urine ( = 1)] isolates were subjected to typing with sequences analysed using BioNumerics version 7.

Results: The sequence was successfully amplified from 77.8% of the strains (154/198) and 47 known types were detected. The distribution of known types in MRSA (36.2%, 17/47) was less diverse than in MSSA (70.2%, 33/47). The most predominant types were t032 (50%) in MRSA, and t127 (19%) and t091 (16.7%) in MSSA, respectively. type t091 in MSSA was significantly associated with skin and soft tissue infections ( = 0.0199).

Conclusion: The previously uncommon type t032 was detected in the Malaysian MRSA strains, which also corresponded to the most common type in Europe and Australia, and has replaced the dominant type t037 which was reported in Malaysia in 2010.

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