Isolation and Analysis of a Symbiosis-regulated and Ras-interacting Vesicular Assembly Protein Gene from the Ectomycorrhizal Fungus Laccaria Bicolor

Overview

Journal New Phytol

Specialty Biology

Date 2021 Apr 20

PMID 33873504

Citations 1

Authors

Sathish Sundaram

Joshua H Brand

Matthew J Hymes

Shivanand Hiremath

Gopi K Podila

Affiliations

Soon will be listed here.

Abstract

• A yeast two-hybrid library prepared from Laccaria bicolor × Pinus resinosa mycorrhizas was screened using a LbRAS clone, previously characterized, as a bait to isolate LbRAS interacting signaling-related genes from L. bicolor. • Using this method, a novel line of Ras-interacting yeast two-hybrid mycorrhizal (Rythm) clones were isolated and analysed for their symbiosis-regulation. One such clone identified (RythmA) had homology to Ap180-like vesicular proteins. • Sequence homology and parsimony-based phylogenetic analysis showed its relatedness to Ap180-like proteins from other systems. DNA analysis suggested that L. bicolor had one or two copies of the RythmA gene. • An RNA analysis showed that the expression of RythmA could be detected 36 h after interaction with the host, which follows the expression of Lbras. Immunolocalization of LbRAS near dolipore septum of the fungal cells in the Hartig net area suggests that RythmA protein may be involved in the transport of signaling proteins such as LbRAS.

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References

Barker , Tagu . The Roles of Auxins and Cytokinins in Mycorrhizal Symbioses. J Plant Growth Regul. 2000; 19(2):144-154. DOI: 10.1007/s003440000021. View

Jane Barker S , Tagu , DELP . Regulation of root and fungal morphogenesis in mycorrhizal symbioses . Plant Physiol. 1998; 116(4):1201-7. PMC: 1539176. DOI: 10.1104/pp.116.4.1201. View

Bendixen C, Gangloff S, Rothstein R . A yeast mating-selection scheme for detection of protein-protein interactions. Nucleic Acids Res. 1994; 22(9):1778-9. PMC: 308069. DOI: 10.1093/nar/22.9.1778. View

Berlocher S, Swofford D . Searching for phylogenetic trees under the frequency parsimony criterion: an approximation using generalized parsimony. Syst Biol. 1997; 46(1):211-5. DOI: 10.1093/sysbio/46.1.211. View

Brodsky F . Living with clathrin: its role in intracellular membrane traffic. Science. 1988; 242(4884):1396-402. DOI: 10.1126/science.2904698. View

De Camilli P, Emr S, McPherson P, Novick P . Phosphoinositides as regulators in membrane traffic. Science. 1996; 271(5255):1533-9. DOI: 10.1126/science.271.5255.1533. View

Fields S, Song O . A novel genetic system to detect protein-protein interactions. Nature. 1989; 340(6230):245-6. DOI: 10.1038/340245a0. View

Fields S, Sternglanz R . The two-hybrid system: an assay for protein-protein interactions. Trends Genet. 1994; 10(8):286-92. DOI: 10.1016/0168-9525(90)90012-u. View

Gavito M, Curtis P, Mikkelsen T, Jakobsen I . Atmospheric CO(2) and mycorrhiza effects on biomass allocation and nutrient uptake of nodulated pea (Pisum sativum L.) plants. J Exp Bot. 2000; 51(352):1931-8. DOI: 10.1093/jexbot/51.352.1931. View

10.

Greener T, Zhao X, Nojima H, Eisenberg E, Greene L . Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells. J Biol Chem. 2000; 275(2):1365-70. DOI: 10.1074/jbc.275.2.1365. View

11.

Hao W, Luo Z, Zheng L, Prasad K, Lafer E . AP180 and AP-2 interact directly in a complex that cooperatively assembles clathrin. J Biol Chem. 1999; 274(32):22785-94. DOI: 10.1074/jbc.274.32.22785. View

12.

Hibbett D, Gilbert L, Donoghue M . Evolutionary instability of ectomycorrhizal symbioses in basidiomycetes. Nature. 2000; 407(6803):506-8. DOI: 10.1038/35035065. View

13.

Jargeat P, Gay G, Debaud J, Marmeisse R . Transcription of a nitrate reductase gene isolated from the symbiotic basidiomycete fungus Hebeloma cylindrosporum does not require induction by nitrate. Mol Gen Genet. 2000; 263(6):948-56. DOI: 10.1007/pl00008695. View

14.

Kholodenko B . Four-dimensional organization of protein kinase signaling cascades: the roles of diffusion, endocytosis and molecular motors. J Exp Biol. 2003; 206(Pt 12):2073-82. DOI: 10.1242/jeb.00298. View

15.

Kim S, Zheng J, Hiremath S, Podila G . Cloning and characterization of a symbiosis-related gene from an ectomycorrhizal fungus Laccaria bicolor. Gene. 1998; 222(2):203-12. DOI: 10.1016/s0378-1119(98)00483-1. View

16.

Kim S, Bernreuther D, Thumm M, Podila G . LB-AUT7, a novel symbiosis-regulated gene from an ectomycorrhizal fungus, Laccaria bicolor, is functionally related to vesicular transport and autophagocytosis. J Bacteriol. 1999; 181(6):1963-7. PMC: 93604. DOI: 10.1128/JB.181.6.1963-1967.1999. View

17.

Lang T, Schaeffeler E, Bernreuther D, Bredschneider M, Wolf D, Thumm M . Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole. EMBO J. 1998; 17(13):3597-607. PMC: 1170696. DOI: 10.1093/emboj/17.13.3597. View

18.

Marsh M, McMahon H . The structural era of endocytosis. Science. 1999; 285(5425):215-20. DOI: 10.1126/science.285.5425.215. View

19.

Moore P, Swords K, Lynch M, Staehelin L . Spatial organization of the assembly pathways of glycoproteins and complex polysaccharides in the Golgi apparatus of plants. J Cell Biol. 1991; 112(4):589-602. PMC: 2288847. DOI: 10.1083/jcb.112.4.589. View

20.

Nehls U, Beguiristain T, Ditengou F, Lapeyrie F, Martin F . The expression of a symbiosis-regulated gene in eucalypt roots is regulated by auxins and hypaphorine, the tryptophan betaine of the ectomycorrhizal basidiomycete Pisolithus tinctorius. Planta. 1999; 207(2):296-302. DOI: 10.1007/s004250050486. View