Chlamydomonas Reinhardtii As a Eukaryotic Photosynthetic Model for Studies of Heavy Metal Homeostasis and Tolerance

Overview

Journal New Phytol

Specialty Biology

Date 2021 Apr 20

PMID 33873346

Citations 15

Authors

M Hanikenne

Affiliations

Soon will be listed here.

Abstract

The green alga Chlamydomonas reinhardtii is a useful model of a photosynthetic cell. This unicellular eukaryote has been intensively used for studies of a number of physiological processes such as photosynthesis, respiration, nitrogen assimilation, flagella motility and basal body function. Its easy-to-manipulate and short life cycle make this organism a powerful tool for genetic analysis. Over the past 15 yr, a dramatically increased number of molecular technologies (including nuclear and organellar transformation systems, cosmid, yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) libraries, reporter genes, RNA interference, DNA microarrays, etc.) have been applied to Chlamydomonas. Moreover, as parts of the Chlamydomonas genome project, molecular mapping, as well as whole genome and extended expressed sequence tag (EST) sequencing programs, are currently underway. These developments have allowed Chlamydomonas to become an extremely valuable model for molecular approaches to heavy metal homeostasis and tolerance in photosynthetic organisms.

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References

Asamizu E, Miura K, Kucho K, Inoue Y, Fukuzawa H, Ohyama K . Generation of expressed sequence tags from low-CO2 and high-CO2 adapted cells of Chlamydomonas reinhardtii. DNA Res. 2000; 7(5):305-7. DOI: 10.1093/dnares/7.5.305. View

Asamizu E, Nakamura Y, Sato S, Fukuzawa H, Tabata S . A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res. 2000; 6(6):369-73. DOI: 10.1093/dnares/6.6.369. View

Ben-Bassat D, Shelef G, Gruner N, Shuval H . Growth of Chlamydomonas in a medium containing mercury. Nature. 1972; 240(5375):43-4. DOI: 10.1038/240043a0. View

Boswell C, Sharma N, Sahi S . Copper tolerance and accumulation potential of Chlamydomonas reinhardtii. Bull Environ Contam Toxicol. 2002; 69(4):546-53. DOI: 10.1007/s00128-002-0096-4. View

Cerutti H, Johnson A, Gillham N, Boynton J . A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics. 1997; 145(1):97-110. PMC: 1207788. DOI: 10.1093/genetics/145.1.97. View

Clemens S . Molecular mechanisms of plant metal tolerance and homeostasis. Planta. 2001; 212(4):475-86. DOI: 10.1007/s004250000458. View

Clemens S, Palmgren M, Kramer U . A long way ahead: understanding and engineering plant metal accumulation. Trends Plant Sci. 2002; 7(7):309-15. DOI: 10.1016/s1360-1385(02)02295-1. View

Cobbett C . Phytochelatin biosynthesis and function in heavy-metal detoxification. Curr Opin Plant Biol. 2000; 3(3):211-6. View

10.

Collard J, Matagne R . Isolation and genetic analysis of Chlamydomonas reinhardtii strains resistant to cadmium. Appl Environ Microbiol. 1990; 56(7):2051-5. PMC: 184559. DOI: 10.1128/aem.56.7.2051-2055.1990. View

11.

Davies J, Yildiz F, Grossman A . Mutants of Chlamydomonas with Aberrant Responses to Sulfur Deprivation. Plant Cell. 1994; 6(1):53-63. PMC: 160415. DOI: 10.1105/tpc.6.1.53. View

12.

Dent R, Han M, Niyogi K . Functional genomics of plant photosynthesis in the fast lane using Chlamydomonas reinhardtii. Trends Plant Sci. 2001; 6(8):364-71. DOI: 10.1016/s1360-1385(01)02018-0. View

13.

Fuhrmann M . Expanding the molecular toolkit for Chlamydomonas reinhardtii--from history to new frontiers. Protist. 2003; 153(4):357-64. DOI: 10.1078/14344610260450082. View

14.

Fuhrmann M, Oertel W, Hegemann P . A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii. Plant J. 1999; 19(3):353-61. DOI: 10.1046/j.1365-313x.1999.00526.x. View

15.

Fuhrmann M, Stahlberg A, Govorunova E, Rank S, Hegemann P . The abundant retinal protein of the Chlamydomonas eye is not the photoreceptor for phototaxis and photophobic responses. J Cell Sci. 2001; 114(Pt 21):3857-63. DOI: 10.1242/jcs.114.21.3857. View

16.

Fujiwara S, Kobayashi I, Hoshino S, Kaise T, Shimogawara K, Usuda H . Isolation and characterization of arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii. Plant Cell Physiol. 2000; 41(1):77-83. DOI: 10.1093/pcp/41.1.77. View

17.

Goyer R . Toxic and essential metal interactions. Annu Rev Nutr. 1997; 17:37-50. DOI: 10.1146/annurev.nutr.17.1.37. View

18.

Grossman A . Chlamydomonas reinhardtii and photosynthesis: genetics to genomics. Curr Opin Plant Biol. 2000; 3(2):132-7. DOI: 10.1016/s1369-5266(99)00053-9. View

19.

Hanikenne M, Matagne R, Loppes R . Pleiotropic mutants hypersensitive to heavy metals and to oxidative stress in Chlamydomonas reinhardtii. FEMS Microbiol Lett. 2001; 196(2):107-11. DOI: 10.1111/j.1574-6968.2001.tb10549.x. View

20.

Harris E . CHLAMYDOMONAS AS A MODEL ORGANISM. Annu Rev Plant Physiol Plant Mol Biol. 2001; 52:363-406. DOI: 10.1146/annurev.arplant.52.1.363. View

21.

Herbik A, Bolling C, Buckhout T . The involvement of a multicopper oxidase in iron uptake by the green algae Chlamydomonas reinhardtii. Plant Physiol. 2002; 130(4):2039-48. PMC: 166715. DOI: 10.1104/pp.013060. View