Effects of Chronic Diabetes Mellitus on the Electrical and Contractile Activities, 45Ca2+ Transport, Fatty Acid Profiles and Ultrastructure of Isolated Rat Ventricular Myocytes

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1988 May 1

PMID 3387190

Citations 8

Authors

M Horackova

M G Murphy

Affiliations

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Abstract

The effects of chronic experimental diabetes on electrophysiological properties, contractile behavior, 45Ca2+ transport, fatty acid profiles and ultrastructural characteristics were studied in enzymatically dissociated ventricular myocytes. Diabetes was induced in rats by streptozotocin administration and animals were killed 8-10 weeks later. Myocytes from diabetic rats exhibited electrical behavior similar to that of myocytes from control rats, but their contractile properties were altered. Their sensitivity of the twitch contractions to various positive and negative inotropic agents (isoproterenol, norepinephrine, phenylephrine, acetylcholine, ouabain and veratridine) was greatly diminished. However, a part of the contractile response (the tonic, sustained contractions) were increased in the diabetic myocytes, indicating that the changes are not caused by a decreased sensitivity of myofilaments. Furthermore, the diabetic myocytes exhibited also significant decrease in total Ca2+ content. The fatty acid profile in the diabetic group was changed mainly in that there were slightly elevated levels of docosahexaenoic acid and diminished levels of palmitic acid. The ultrastructure of the diabetic myocytes was affected only slightly. These investigations offer for the first time a comprehensive picture of changes related to diabetic cardiomyopathy as they occur at the level of cardiomyocytes.

Citing Articles

Electrical Features of the Diabetic Myocardium. Arrhythmic and Cardiovascular Safety Considerations in Diabetes.

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Alterations in sarcoplasmic reticulum and mitochondrial functions in diabetic cardiomyopathy.

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Effects of rosiglitazone on altered electrical left ventricular papillary muscle activities of diabetic rat.

Kavak S, Emre M, Tetiker T, Kavak T, Kolcu Z, Gunay I Naunyn Schmiedebergs Arch Pharmacol. 2007; 376(6):415-21.

PMID: 18097651 DOI: 10.1007/s00210-007-0234-y.

Inhibition of nitric oxide synthase enhances contractile response of ventricular myocytes from streptozotocin-diabetic rats.

Smith J, Sondgeroth K, Wahler G Mol Cell Biochem. 2007; 300(1-2):129-37.

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Altered cardiac adrenergic neurotransmission in streptozotocin-induced diabetic rats.

Gando S, Hattori Y, Kanno M Br J Pharmacol. 1993; 109(4):1276-81.

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