Epigenetic Silencing of and Genes by CpG Island Hypermethylation in Epithelial Ovarian Cancer Patients

Overview

Journal Indian J Clin Biochem

Specialty Biochemistry

Date 2021 Apr 19

PMID 33867711

Citations 3

Authors

Mariyam Zuberi

Sagar Dholariya

Imran Khan

Rashid Mir

Sameer Guru

Musadiq Bhat

Mamta Sumi

Alpana Saxena

Affiliations

Soon will be listed here.

Abstract

Transcriptional silencing induced by hypermethylation of CpG islands in the promoter regions of genes is believed to be an important mechanism of carcinogenesis in human cancers including epithelial ovarian cancer (EOC). Previously published data on gene methylation of EOC focused mainly on single gene or on cancer tissues. Objectives of the study were to estimate the promoter hypermethylation status of and genes in circulating blood of EOC patients and to determine their association with clinicopathological features of EOC. This case-control study included 50 EOC patients and 20 apparently healthy and age matched female controls. Isolation of genomic DNA was carried out from peripheral venous blood. Methylation in promoter region of and genes was determined by methylation-specific PCR. Methylation of was occurred in 42 out of 50 cases (84.0%) and methylation of gene was occurred in 34 out of 50 cases (68.0%). Methylation of both genes was occurred in 25 cases (50.0%). Occurrence of methylation in and genes was statistically significant ( < 0.0001) in cases compared to controls. Methylation of both genes was not statistically associated with age at diagnosis, menopausal status, histopathological types and FIGO staging of EOC. Identification of the peculiar promoter hypermethylation of and genes might be a successful approach for ancillary diagnosis of EOC at early stage in blood sample.

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