Occurrence and Antimicrobial Susceptibility Profile of Isolates from Animal Origin Food Items in Selected Areas of Arsi Zone, Southeastern Ethiopia, 2018/19

Overview

Journal Int J Microbiol

Publisher Wiley

Specialty Microbiology

Date 2021 Apr 16

PMID 33859696

Citations 6

Authors

Minda Asfaw Geresu

Behailu Assefa Wayuo

Gezahegne Mamo Kassa

Affiliations

Soon will be listed here.

Abstract

The status of and its antimicrobial susceptibility profile in animal origin food items from different catering establishments in Ethiopia is scarce. Hence, this study aimed to investigate the occurrence and antimicrobial susceptibility profile of isolates from animal origin food items in the selected areas of Arsi Zone. One hundred ninety-two animal origin food samples were collected and processed for isolation. Isolates were tested for their susceptibility to 13 antimicrobials using Kirby-Bauer disk diffusion assay. An overall prevalence of 9.4% (18/192) spp. isolates were recovered from animal origin food samples collected from different catering establishments. Seven (21.9%) of 4 (12.5%) of 3 (9.4%) of 2 (6.3%) of raw milk, 1 (3.1%) of egg sandwich and 1 (3.1%) of cream cake samples were positive for Catering establishments, protective clothing, source of contamination, manner of hand washing, and money handling were among the putative risk factors that were significantly associated ( < 0.05) with spp. occurrence. Ampicillin, nitrofurans, and sulphonamide resistance were significantly associated ( < 0.05) with spp. occurrence in the selected food items. Three (16.7%), 5 (27.8%), 5 (27.8%), and 4 (22.2%) of the isolates were resistant to 3, 4, 5, and 6 antibiotics, respectively, whereas only a sole isolate was resistant to two antibiotics (viz. ampicillin and kanamycin). In conclusion, the general sanitary condition of the catering establishments, utensils used, and personnel hygienic practices were not to the recommended standards in the current study. Besides, detection of multidrug-resistant strains of in animal origin food items from different catering establishments suggests the need for detailed epidemiological and molecular characterization of the pathogen so as to establish the sources of acquisition of resistant strains. Hence, implementation of prevention and control strategies from farm production to consumption of animal origin food items are crucial.

Citing Articles

Frequency and antibiotic resistance pattern of spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran.

Barzegar-Bafrouei R, Hajimohammadi B, Zandi H, Eslami G, Fallahzadeh H Iran J Microbiol. 2024; 16(6):755-762.

PMID: 39737352 PMC: 11682549. DOI: 10.18502/ijm.v16i6.17248.

Occurrence and antimicrobial susceptibility of Salmonella enterica in milk along the supply chain, humans, and the environment in Woliata Sodo, Ethiopia.

Ayichew S, Zewdu A, Megerrsa B, Sori T, Gutema F BMC Microbiol. 2024; 24(1):538.

PMID: 39716083 PMC: 11665056. DOI: 10.1186/s12866-024-03689-4.

Prevalence, Molecular Detection, and Antimicrobial Resistance of Isolates from Poultry Farms across Central Ethiopia: A Cross-Sectional Study in Urban and Peri-Urban Areas.

Waktole H, Ayele Y, Ayalkibet Y, Teshome T, Muluneh T, Ayane S Microorganisms. 2024; 12(4).

PMID: 38674711 PMC: 11051739. DOI: 10.3390/microorganisms12040767.

Camel Milk: Antimicrobial Agents, Fermented Products, and Shelf Life.

Hamed N, Mbye M, Ayyash M, Ulusoy B, Kamal-Eldin A Foods. 2024; 13(3).

PMID: 38338516 PMC: 10855775. DOI: 10.3390/foods13030381.

A scoping review of zoonotic parasites and pathogens associated with abattoirs in Eastern Africa and recommendations for abattoirs as disease surveillance sites.

Rodarte K, Fair J, Bett B, Kerfua S, Fasina F, Bartlow A Front Public Health. 2023; 11:1194964.

PMID: 37529427 PMC: 10387540. DOI: 10.3389/fpubh.2023.1194964.

References

Galgallo D, Roka Z, Boru W, Abill K, Ransom J . Investigation of a typhoid fever epidemic in Moyale Sub-County, Kenya, 2014-2015. J Health Popul Nutr. 2018; 37(1):14. PMC: 5952430. DOI: 10.1186/s41043-018-0144-2. View

Vindigni S, Srijan A, Wongstitwilairoong B, Marcus R, Meek J, Riley P . Prevalence of foodborne microorganisms in retail foods in Thailand. Foodborne Pathog Dis. 2007; 4(2):208-15. DOI: 10.1089/fpd.2006.0077. View

Wang X, Biswas S, Paudyal N, Pan H, Li X, Fang W . Antibiotic Resistance in Typhimurium Isolates Recovered From the Food Chain Through National Antimicrobial Resistance Monitoring System Between 1996 and 2016. Front Microbiol. 2019; 10:985. PMC: 6514237. DOI: 10.3389/fmicb.2019.00985. View

Tadesse G . A meta-analysis of the proportion of animal Salmonella isolates resistant to drugs used against human salmonellosis in Ethiopia. BMC Infect Dis. 2015; 15:84. PMC: 4352553. DOI: 10.1186/s12879-015-0835-x. View

Diriba K, Awulachew E, Ashuro Z . Prevalence and Antimicrobial Resistance Pattern of , and Intestinal Parasites and Associated Factor among Food Handlers in Dilla University Student Cafeteria, Dilla, Ethiopia. Int J Microbiol. 2020; 2020:3150539. PMC: 7292009. DOI: 10.1155/2020/3150539. View

Eguale T, Gebreyes W, Asrat D, Alemayehu H, Gunn J, Engidawork E . Non-typhoidal Salmonella serotypes, antimicrobial resistance and co-infection with parasites among patients with diarrhea and other gastrointestinal complaints in Addis Ababa, Ethiopia. BMC Infect Dis. 2015; 15:497. PMC: 4634906. DOI: 10.1186/s12879-015-1235-y. View

Paudyal N, Anihouvi V, Hounhouigan J, Matsheka M, Sekwati-Monang B, Amoa-Awua W . Prevalence of foodborne pathogens in food from selected African countries - A meta-analysis. Int J Food Microbiol. 2017; 249:35-43. DOI: 10.1016/j.ijfoodmicro.2017.03.002. View

Eguale T . Non-typhoidal Salmonella serovars in poultry farms in central Ethiopia: prevalence and antimicrobial resistance. BMC Vet Res. 2018; 14(1):217. PMC: 6035434. DOI: 10.1186/s12917-018-1539-4. View

Garedew L, Hagos Z, Addis Z, Tesfaye R, Zegeye B . Prevalence and antimicrobial susceptibility patterns of Salmonella isolates in association with hygienic status from butcher shops in Gondar town, Ethiopia. Antimicrob Resist Infect Control. 2015; 4:21. PMC: 4480883. DOI: 10.1186/s13756-015-0062-7. View

10.

Ejo M, Garedew L, Alebachew Z, Worku W . Prevalence and Antimicrobial Resistance of Isolated from Animal-Origin Food Items in Gondar, Ethiopia. Biomed Res Int. 2017; 2016:4290506. PMC: 5198084. DOI: 10.1155/2016/4290506. View

11.

Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes W, Gunn J . Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018; 65(7):766-776. DOI: 10.1111/zph.12490. View

12.

Bersisa A, Tulu D, Negera C . Investigation of Bacteriological Quality of Meat from Abattoir and Butcher Shops in Bishoftu, Central Ethiopia. Int J Microbiol. 2019; 2019:6416803. PMC: 6525944. DOI: 10.1155/2019/6416803. View

13.

Mather A, Reid S, Maskell D, Parkhill J, Fookes M, Harris S . Distinguishable epidemics of multidrug-resistant Salmonella Typhimurium DT104 in different hosts. Science. 2013; 341(6153):1514-7. PMC: 4012302. DOI: 10.1126/science.1240578. View

14.

Faour-Klingbeil D, Todd E . Prevention and Control of Foodborne Diseases in Middle-East North African Countries: Review of National Control Systems. Int J Environ Res Public Health. 2019; 17(1). PMC: 6982137. DOI: 10.3390/ijerph17010070. View

15.

Molla W, Molla B, Alemayehu D, Muckle A, Cole L, Wilkie E . Occurrence and antimicrobial resistance of Salmonella serovars in apparently healthy slaughtered sheep and goats of central Ethiopia. Trop Anim Health Prod. 2007; 38(6):455-62. DOI: 10.1007/s11250-006-4325-4. View

16.

Garedew L, Berhanu A, Mengesha D, Tsegay G . Identification of gram-negative bacteria from critical control points of raw and pasteurized cow milk consumed at Gondar town and its suburbs, Ethiopia. BMC Public Health. 2012; 12:950. PMC: 3527359. DOI: 10.1186/1471-2458-12-950. View

17.

Kumalo A, Gambura E, Dodicho T, Ahmed K, Balcha T, Beshir B . Prevalence of Intestinal Parasites and among Food Handlers Working in Catering Establishments of Public Institutes Found in Dawuro Zone, South-Western Ethiopia. J Parasitol Res. 2021; 2021:8889302. PMC: 7826225. DOI: 10.1155/2021/8889302. View

18.

Wabeto W, Abraham Y, Anjulo A . Detection and identification of antimicrobial-resistant Salmonella in raw beef at Wolaita Sodo municipal abattoir, Southern Ethiopia. J Health Popul Nutr. 2017; 36(1):52. PMC: 5732392. DOI: 10.1186/s41043-017-0131-z. View

19.

Stevens A, Kabore Y, Perrier-Gros-Claude J, Millemann Y, Brisabois A, Catteau M . Prevalence and antibiotic-resistance of Salmonella isolated from beef sampled from the slaughterhouse and from retailers in Dakar (Senegal). Int J Food Microbiol. 2006; 110(2):178-86. DOI: 10.1016/j.ijfoodmicro.2006.04.018. View

20.

Takele S, Woldemichael K, Gashaw M, Tassew H, Yohannes M, Abdissa A . Prevalence and drug susceptibility pattern of isolates from apparently healthy slaughter cattle and personnel working at the Jimma municipal abattoir, south-West Ethiopia. Trop Dis Travel Med Vaccines. 2018; 4:13. PMC: 6154828. DOI: 10.1186/s40794-018-0072-6. View