Antimicrobial Susceptibility, Serotype Distribution, Virulence Profile and Molecular Typing of Piliated Clinical Isolates of Pneumococci from East Coast, Peninsular Malaysia

Overview

Journal Sci Rep

Specialty Science

Date 2021 Apr 16

PMID 33859249

Citations 8

Authors

Nurul Diana Dzaraly

Mohd Nasir Mohd Desa

AbdulRahman Muthanna

Siti Norbaya Masri

Niazlin Mohd Taib

Zarizal Suhaili

Nurshahira Sulaiman

Nurul Hana Zainal Baharin

Cheah Yun Shuan

Zarina Ariffin

Nor Iza A Rahman

Farahiyah Mohd Rani

Navindra Kumari Palanisamy

Tuan Suhaila Tuan Soh

Fatimah Haslina Abdullah

Affiliations

Soon will be listed here.

Abstract

Pilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, including the piliated isolates, in relation to antimicrobial susceptibility, serotypes, and genotypes at a major tertiary hospital on the east coast of Peninsular Malaysia. A total of 100 clinical isolates collected between September 2017 and December 2019 were subjected to serotyping, antimicrobial susceptibility test, and detection of pneumococcal virulence and pilus genes. Multilocus sequence typing (MLST) and phylogenetic analysis were performed only for piliated strains. The most frequent serotypes were 14 (17%), 6A/B (16%), 23F (12%), 19A (11%), and 19F (11%). The majority of isolates were resistant to erythromycin (42%), tetracycline (37%), and trimethoprim-sulfamethoxazole (24%). Piliated isolates occurred in a proportion of 19%; 47.3% of them were multidrug-resistant (MDR) and a majority had serotype 19F. This study showed ST236 was the most predominant sequence type (ST) among piliated isolates, which was related to PMEN clone Taiwan-14 (CC271). In the phylogenetic analysis, the piliated isolates were grouped into three major clades supported with 100% bootstrap values. Most piliated isolates belonged to internationally disseminated clones of S. pneumoniae, but pneumococcal conjugate vaccines (PCVs) have the potential to control them.

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References

Kreikemeyer B, Gamez G, Margarit I, Giard J, Hammerschmidt S, Hartke A . Genomic organization, structure, regulation and pathogenic role of pilus constituents in major pathogenic Streptococci and Enterococci. Int J Med Microbiol. 2010; 301(3):240-51. DOI: 10.1016/j.ijmm.2010.09.003. View

Zahner D, Gudlavalleti A, Stephens D . Increase in pilus islet 2-encoded pili among Streptococcus pneumoniae isolates, Atlanta, Georgia, USA. Emerg Infect Dis. 2010; 16(6):955-62. PMC: 3086225. DOI: 10.3201/eid1606.091820. View

Aguiar S, Serrano I, Pinto F, Melo-Cristino J, Ramirez M . The presence of the pilus locus is a clonal property among pneumococcal invasive isolates. BMC Microbiol. 2008; 8:41. PMC: 2270847. DOI: 10.1186/1471-2180-8-41. View

Kawaguchiya M, Urushibara N, Aung M, Shinagawa M, Takahashi S, Kobayashi N . Serotype distribution, antimicrobial resistance and prevalence of pilus islets in pneumococci following the use of conjugate vaccines. J Med Microbiol. 2017; 66(5):643-650. DOI: 10.1099/jmm.0.000479. View

Enright M, Spratt B . A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology (Reading). 1998; 144 ( Pt 11):3049-3060. DOI: 10.1099/00221287-144-11-3049. View

Hjalmarsdottir M, Petursdottir B, Erlendsdottir H, Haraldsson G, Kristinsson K . Prevalence of pilus genes in pneumococci isolated from healthy preschool children in Iceland: association with vaccine serotypes and antibiotic resistance. J Antimicrob Chemother. 2015; 70(8):2203-8. DOI: 10.1093/jac/dkv096. View

Vorobieva S Jensen V, Furberg A, Slotved H, Bazhukova T, Haldorsen B, Caugant D . Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines. BMC Infect Dis. 2020; 20(1):279. PMC: 7161136. DOI: 10.1186/s12879-020-04998-5. View

Arushothy R, Ahmad N, Amran F, Hashim R, Samsudin N, Azih C . Pneumococcal serotype distribution and antibiotic susceptibility in Malaysia: A four-year study (2014-2017) on invasive paediatric isolates. Int J Infect Dis. 2018; 80:129-133. DOI: 10.1016/j.ijid.2018.12.009. View

Subramaniam P, Abdul Jabar K, Kee B, Chong C, Nathan A, de Bruyne J . Serotypes & penicillin susceptibility of isolated from children admitted to a tertiary teaching hospital in Malaysia. Indian J Med Res. 2018; 148(2):225-231. PMC: 6206763. DOI: 10.4103/ijmr.IJMR_1987_16. View

10.

Dzaraly N, Muthanna A, Desa M, Mohd Taib N, Masri S, A Rahman N . Pilus islets and the clonal spread of piliated Streptococcus pneumoniae: A review. Int J Med Microbiol. 2020; 310(7):151449. DOI: 10.1016/j.ijmm.2020.151449. View

11.

Shakrin N, Balasubramaniam S, Yusof H, Mastuki M, Masri S, Taib N . Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae. Trop Biomed. 2013; 30(2):338-44. View

12.

Lo S, Gladstone R, van Tonder A, Lees J, du Plessis M, Benisty R . Pneumococcal lineages associated with serotype replacement and antibiotic resistance in childhood invasive pneumococcal disease in the post-PCV13 era: an international whole-genome sequencing study. Lancet Infect Dis. 2019; 19(7):759-769. PMC: 7641901. DOI: 10.1016/S1473-3099(19)30297-X. View

13.

Ganaie F, Saad J, McGee L, van Tonder A, Bentley S, Lo S . A New Pneumococcal Capsule Type, 10D, is the 100th Serotype and Has a Large Fragment from an Oral Streptococcus. mBio. 2020; 11(3). PMC: 7240158. DOI: 10.1128/mBio.00937-20. View

14.

Knupp-Pereira P, Marques N, Teixeira L, Povoa H, Neves F . Prevalence of PspA families and pilus islets among Streptococcus pneumoniae colonizing children before and after universal use of pneumococcal conjugate vaccines in Brazil. Braz J Microbiol. 2019; 51(2):419-425. PMC: 7203294. DOI: 10.1007/s42770-019-00179-y. View

15.

Wang X, Cong Z, Huang W, Li C . Molecular characterization of Streptococcus pneumoniae isolated from pediatric patients in Shanghai, China. Pediatr Pulmonol. 2020; 55(8):2135-2141. DOI: 10.1002/ppul.24877. View

16.

Seki M, Yamashita Y, Torigoe H, Tsuda H, Sato S, Maeno M . Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae. J Clin Microbiol. 2005; 43(4):1581-6. PMC: 1081357. DOI: 10.1128/JCM.43.4.1581-1586.2005. View

17.

Safari D, Valentiya F, Salsabila K, Paramaiswari W, Tafroji W, Hammerschmidt S . The prevalence of pilus islets in isolates from healthy children in Indonesia. Access Microbiol. 2021; 3(1):acmi000184. PMC: 8115978. DOI: 10.1099/acmi.0.000184. View

18.

Fu J, Li L, Liang Z, Xu S, Lin N, Qin P . Etiology of acute otitis media and phenotypic-molecular characterization of Streptococcus pneumoniae isolated from children in Liuzhou, China. BMC Infect Dis. 2019; 19(1):168. PMC: 6377742. DOI: 10.1186/s12879-019-3795-8. View

19.

Sheppard C, Harrison T, Morris R, Hogan A, George R . Autolysin-targeted LightCycler assay including internal process control for detection of Streptococcus pneumoniae DNA in clinical samples. J Med Microbiol. 2004; 53(Pt 3):189-195. DOI: 10.1099/jmm.0.05460-0. View

20.

Magiorakos A, Srinivasan A, Carey R, Carmeli Y, Falagas M, Giske C . Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2011; 18(3):268-81. DOI: 10.1111/j.1469-0691.2011.03570.x. View