Activation of RAW264.7 Cells by PCp-I, a Polysaccharide from L, Through NF-B/MAPK Signalling Pathway

Overview

Journal Int J Immunopathol Pharmacol

Publisher Sage Publications

Specialties Allergy & Immunology
Pathology
Pharmacology

Date 2021 Apr 15

PMID 33855900

Citations 6

Authors

Honglin Wang

Xiaoqing Xu

Zhenhua Yin

Mengke Wang

Baoguang Wang

Changyang Ma

Jinmei Wang

Wenyi Kang

Affiliations

Soon will be listed here.

Abstract

PCp-I is a polysaccharide isolated and identified from the L. by our research group. In this study, the immunomodulatory effects of PCp-I on RAW264.7 cells was evaluated. PCp-I could enhance the level of NO along with up-regulation of iNOS mRNA in RAW264.7 cells. The PCp-I could significantly up-regulate the mRNA expression of TNF- and IL-6 in RAW264.7 cells, and then the expression of TNF-, IL-6, ROS and the phagocytic activity were increased. Additionally, PCp-I could significantly up-regulate the phosphorylation level of p65, p38, ERK and JNK proteins, which proved that PCp-I could activate the macrophages by MAPKs and NF-B signalling pathway and the TLR4 may be one of the receptors of PCp-I regulate the RAW264.7 cells.

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References

Baeuerle P, Henkel T . Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994; 12:141-79. DOI: 10.1146/annurev.iy.12.040194.001041. View

Scheller J, Chalaris A, Schmidt-Arras D, Rose-John S . The pro- and anti-inflammatory properties of the cytokine interleukin-6. Biochim Biophys Acta. 2011; 1813(5):878-88. DOI: 10.1016/j.bbamcr.2011.01.034. View

Cheng X, Wu Q, Zhao J, Su T, Lu Y, Zhang W . Immunomodulatory effect of a polysaccharide fraction on RAW 264.7 macrophages extracted from the wild Lactarius deliciosus. Int J Biol Macromol. 2019; 128:732-739. DOI: 10.1016/j.ijbiomac.2019.01.201. View

Beutler B, Hoebe K, Du X, Ulevitch R . How we detect microbes and respond to them: the Toll-like receptors and their transducers. J Leukoc Biol. 2003; 74(4):479-85. DOI: 10.1189/jlb.0203082. View

Karin M . The regulation of AP-1 activity by mitogen-activated protein kinases. J Biol Chem. 1995; 270(28):16483-6. DOI: 10.1074/jbc.270.28.16483. View

Ferreira S, Passos C, Madureira P, Vilanova M, Coimbra M . Structure-function relationships of immunostimulatory polysaccharides: A review. Carbohydr Polym. 2015; 132:378-96. DOI: 10.1016/j.carbpol.2015.05.079. View

Neurath M . Cytokines in inflammatory bowel disease. Nat Rev Immunol. 2014; 14(5):329-42. DOI: 10.1038/nri3661. View

Xu X, Wu X, Wang Q, Cai N, Zhang H, Jiang Z . Immunomodulatory Effects of Alginate Oligosaccharides on Murine Macrophage RAW264.7 Cells and Their Structure-Activity Relationships. J Agric Food Chem. 2014; 62(14):3168-3176. DOI: 10.1021/jf405633n. View

Liu J, Yang F, Lu C, Yang Y, Wen C, Hua K . Polysaccharides from Dioscorea batatas induce tumor necrosis factor-alpha secretion via Toll-like receptor 4-mediated protein kinase signaling pathways. J Agric Food Chem. 2008; 56(21):9892-8. DOI: 10.1021/jf8018722. View

10.

Yu Q, Nie S, Wang J, Yin P, Li W, Xie M . Polysaccharide from Ganoderma atrum induces tumor necrosis factor-α secretion via phosphoinositide 3-kinase/Akt, mitogen-activated protein kinase and nuclear factor-κB signaling pathways in RAW264.7 cells. Int Immunopharmacol. 2012; 14(4):362-8. DOI: 10.1016/j.intimp.2012.09.005. View

11.

You L, Gao Q, Feng M, Yang B, Ren J, Gu L . Structural characterisation of polysaccharides from Tricholoma matsutake and their antioxidant and antitumour activities. Food Chem. 2013; 138(4):2242-9. DOI: 10.1016/j.foodchem.2012.11.140. View

12.

Vijay K . Toll-like receptors in immunity and inflammatory diseases: Past, present, and future. Int Immunopharmacol. 2018; 59:391-412. PMC: 7106078. DOI: 10.1016/j.intimp.2018.03.002. View

13.

Aderem A . Phagocytosis and the inflammatory response. J Infect Dis. 2003; 187 Suppl 2:S340-5. DOI: 10.1086/374747. View

14.

ODea E, Hoffmann A . The regulatory logic of the NF-kappaB signaling system. Cold Spring Harb Perspect Biol. 2010; 2(1):a000216. PMC: 2827908. DOI: 10.1101/cshperspect.a000216. View

15.

An H, Xu H, Yu Y, Zhang M, Qi R, Yan X . Up-regulation of TLR9 gene expression by LPS in mouse macrophages via activation of NF-kappaB, ERK and p38 MAPK signal pathways. Immunol Lett. 2002; 81(3):165-9. DOI: 10.1016/s0165-2478(02)00010-x. View

16.

Bosca L, Zeini M, Traves P, Hortelano S . Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005; 208(2):249-58. DOI: 10.1016/j.tox.2004.11.035. View

17.

Wang Z, Xie J, Kan L, Wang J, Shen M, Li W . Sulfated polysaccharides from Cyclocarya paliurus reduce H2O2-induced oxidative stress in RAW264.7 cells. Int J Biol Macromol. 2015; 80:410-7. DOI: 10.1016/j.ijbiomac.2015.06.031. View

18.

Kim H, Kim J, Kang J, Kim H, Kim Y, Hong I . Cordlan polysaccharide isolated from mushroom Cordyceps militaris induces dendritic cell maturation through toll-like receptor 4 signalings. Food Chem Toxicol. 2010; 48(7):1926-33. DOI: 10.1016/j.fct.2010.04.036. View

19.

Sakurai H, Chiba H, Miyoshi H, Sugita T, Toriumi W . IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain. J Biol Chem. 1999; 274(43):30353-6. DOI: 10.1074/jbc.274.43.30353. View

20.

Wei W, Xiao H, Bao W, Ma D, Leung C, Han X . TLR-4 may mediate signaling pathways of Astragalus polysaccharide RAP induced cytokine expression of RAW264.7 cells. J Ethnopharmacol. 2016; 179:243-52. DOI: 10.1016/j.jep.2015.12.060. View