The ID NOW COVID-19, a High-speed High-performance Assay

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2021 Apr 15

PMID 33855651

Citations 7

Authors

Eric Farfour

M Asso-Bonnet

M Vasse

Affiliations

Soon will be listed here.

Abstract

The ID NOW COVID-19 assay is a promising tool for the rapid identification of COVID-19 patients. However, its performances were questioned. We evaluate the ID NOW COVID-19 in comparison to a reference RT-PCR using a collection of 48 fresh nasopharyngeal swabs sampled on universal transport media (UTM). Only 2 false negatives of the ID NOW COVID-19 were identified. They display PCR cycle threshold values of 37.5 and 39.2. The positive percent agreement and the negative percent agreement were 94.9% and 100%, respectively. The Kappa value was 0.88. The ID NOW COVID-19 combines high-speed and accurate processing. Using UTM, the ID NOW COVID-19 could be repeated in the case of invalid result. Further analyses, such as screening of genetic variants or genome sequencing, could also be performed with the same sample. As for all tests, the results should be interpreted according to clinical and epidemiological context.

Citing Articles

Molecular characterization of SARS-CoV-2 nucleocapsid protein.

Huang Y, Chen J, Chen S, Huang C, Li B, Li J Front Cell Infect Microbiol. 2024; 14:1415885.

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Rapid Nucleic Acid Diagnostic Technology for Pandemic Diseases.

Lei Y, Xu D Molecules. 2024; 29(7).

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The role of SARS-CoV-2 N protein in diagnosis and vaccination in the context of emerging variants: present status and prospects.

Song W, Fang Z, Ma F, Li J, Huang Z, Zhang Y Front Microbiol. 2023; 14:1217567.

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Comparison of Abbott ID NOW, a novel isothermal amplification based COVID-19 diagnostic method with RTPCR.

Srivastava S, Singh P, Malhotra R, Mathur P J Virol Methods. 2022; 304:114521.

PMID: 35278535 PMC: 8906026. DOI: 10.1016/j.jviromet.2022.114521.

Comparative Evaluation of Cartridge-Based Abbott ID NOW Test With Probe-Based Real-Time Reverse Transcription Polymerase Chain Reaction Assay for Detection of SARS-CoV-2.

Pattnaik D, Poddar N, Pathi B, Panigrahi K, Sabat S, Roy A Cureus. 2022; 14(2):e22470.

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