Facile Method for Morphological Characterization at Nano Scale

Overview

Journal Iran J Biotechnol

Specialty Biotechnology

Date 2021 Apr 14

PMID 33850948

Citations 2

Authors

Ali Dinari

Seyedeh Sahar Mortazavi Farsani

Soheila Mohammadi

Farhood Najafi

Mahdi Abdollahi

Affiliations

Soon will be listed here.

Abstract

Background: Dynamic light scattering (DLS) and electron microscopy (EM) are the most practical techniques for nanoparticles (NPs) characterization. However, the impediments which involved the sample preparation method lead to failure in provided results of mentioned device analysis. These problems will be intensifying, if the examined samples are the soft nanocarriers such as organic ones or biological samples.

Objectives: In order to achieve the appropriate results from DLS and EM analysis, an optimized protocol was introduced by this research which would prepare samples with high degree of quality and accuracy.

Materials And Methods: Morphological analysis of prepared polymeric nanocarriers (micelles, nanogels) by this protocol were done. Filtration, dilution and sonication as three crucial and effectiveness steps of sample preparation were assessed through DLS data and EM images.

Results: This research has tried to introduce a facile method with novelty of simplicity and rapidity. These triple steps could improve the quality of morphological data. The obtained results indicated that sample preparation methods have the most effective factors on sample size distribution and homogeneity of desired samples.

Conclusion: The suggested optimized preparation method will be helpful for all soft nanomaterial's samples.

Citing Articles

Polymeric Micro/Nanocarriers and Motors for Cargo Transport and Phototriggered Delivery.

Mena-Giraldo P, Orozco J Polymers (Basel). 2021; 13(22).

PMID: 34833219 PMC: 8621231. DOI: 10.3390/polym13223920.

Design and fabrication of dual responsive lignin-based nanogel via "grafting from" atom transfer radical polymerization for curcumin loading and release.

Dinari A, Abdollahi M, Sadeghizadeh M Sci Rep. 2021; 11(1):1962.

PMID: 33479381 PMC: 7820611. DOI: 10.1038/s41598-021-81393-3.

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