Evaluation of the Spanish Population Coverage of a Prospective HLA Haplobank of Induced Pluripotent Stem Cells

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2021 Apr 14

PMID 33849662

Citations 11

Authors

Belen Alvarez-Palomo

Iris Garcia-Martinez

Jorge Gayoso

Angel Raya

Anna Veiga

Maria Luisa Abad

Adolfo Eiras

Maria Guzman-Fulgencio

Mar Luis-Hidalgo

Cristina Eguizabal

Silvia Santos

Antonio Balas

Raquel Alenda

Francisco Sanchez-Gordo

Laura Ponce Verdugo

Juliana Villa

Enric Carreras

Francisco Vidal

Alejandro Madrigal

Maria Jose Herrero

Francesc Rudilla

Sergi Querol

Affiliations

Soon will be listed here.

Abstract

Background: iPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplantation of iPSC derived cell therapies. Cord blood banks offer an extensive source of HLA-typed cells suitable for reprogramming to iPSC. Several initiatives worldwide have been undertaken to create national and international iPSC haplobanks that match a significant part of a population.

Methods: To create an iPSC haplobank that serves the Spanish population (IPS-PANIA), we have searched the Spanish Bone Marrow Donor Registry (REDMO) to identify the most frequently estimated haplotypes. From the top ten donors identified, we estimated the population coverage using the criteria of zero mismatches in HLA-A, HLA-B, and HLA-DRB1 with different stringencies: high resolution, low resolution, and beneficial mismatch.

Results: We have calculated that ten cord blood units from homozygous donors stored at the Spanish cord blood banks can provide HLA-A, HLA-B, and HLA-DRB1 matching for 28.23% of the population.

Conclusion: We confirm the feasibility of using banked cord blood units to create an iPSC haplobank that will cover a significant percentage of the Spanish and international population for future advanced therapy replacement strategies.

Citing Articles

Constructing a potential HLA haplo-homozygous induced pluripotent stem cell haplobank using data from an umbilical cord blood bank.

He J, He Y, Zhan Q, Wu Z, Sheng Q, Zhang W Stem Cell Res Ther. 2025; 16(1):42.

PMID: 39901240 PMC: 11792705. DOI: 10.1186/s13287-025-04159-y.

Current Landscape of iPSC Haplobanks.

Escriba R, Beksac M, Bennaceur-Griscelli A, Glover J, Koskela S, Latsoudis H Stem Cell Rev Rep. 2024; 20(8):2155-2164.

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Induced pluripotent stem cells (iPSCs): molecular mechanisms of induction and applications.

Cerneckis J, Cai H, Shi Y Signal Transduct Target Ther. 2024; 9(1):112.

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Perspectives for the Use of Umbilical Cord Blood in Transplantation and Beyond: Initiatives for an Advanced and Sustainable Public Banking Program in Greece.

Pateraki P, Latsoudis H, Papadopoulou A, Gontika I, Fragiadaki I, Mavroudi I J Clin Med. 2024; 13(4).

PMID: 38398465 PMC: 10889829. DOI: 10.3390/jcm13041152.

HLA-based banking of induced pluripotent stem cells in Saudi Arabia.

Alowaysi M, Lehmann R, Al-Shehri M, Baadhaim M, Alzahrani H, Aboalola D Stem Cell Res Ther. 2023; 14(1):374.

PMID: 38111036 PMC: 10729375. DOI: 10.1186/s13287-023-03612-0.

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