Characterization of a Monoclonal Antibody Against Infective Larvae of Brugia Malayi

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1988 May 1

PMID 3384450

Citations 4

Authors

P B Parab

G R Rajasekariah

R Chandrashekar

S S Alkan

D G Braun

D Subrahmanyam

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies were produced following immunization of mice with live infective larvae of Brugia malayi. One of these, 46.08.76, is an antibody that promotes adherence of mouse peritoneal macrophages and human peripheral blood leucocytes to the infective larvae of B. malayi and Wuchereria bancrofti, respectively, and kills them. Fresh normal serum, as a source of complement, augments this effect. The same monoclonal antibody conferred 89% protection to jirds (Meriones unguiculatus) against challenge infection of B. malayi stage-three larvae. This monoclonal antibody recognizes antigens of 80,000, 67,000, 52,000 and 36,000 MW proteins present among the antigens of larvae, as detected by an immunoblotting technique. The antibody also reacts with antigens of infective larvae of Litomosoides carinii, Dipetalonema viteae and B. pahangi, but to a smaller extent.

Citing Articles

Monoclonal Antibodies Targeting an Extracellular Vesicle Tetraspanin Protect Hamsters against Challenge Infection.

Phumrattanaprapin W, Pearson M, Pickering D, Tedla B, Smout M, Chaiyadet S Vaccines (Basel). 2021; 9(7).

PMID: 34358156 PMC: 8310160. DOI: 10.3390/vaccines9070740.

Functions of Antibodies.

Forthal D Microbiol Spectr. 2014; 2(4):1-17.

PMID: 25215264 PMC: 4159104.

To B or not to B: B cells and the Th2-type immune response to helminths.

Harris N, Gause W Trends Immunol. 2010; 32(2):80-8.

PMID: 21159556 PMC: 3076625. DOI: 10.1016/j.it.2010.11.005.

Protective immune responses with trickle infections of third-stage filarial larvae of Wuchereria bancrofti in mice.

Rajasekariah G, Monteiro Y, Netto A, Deshpande L, Subrahmanyam D Clin Exp Immunol. 1989; 78(2):292-8.

PMID: 12412764 PMC: 1534677.

References

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Galfre G, Howe S, Milstein C, Butcher G, Howard J . Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977; 266(5602):550-2. DOI: 10.1038/266550a0. View

Subrahmanyam D, Mehta K, Nelson D, Rao Y, RAO C . Immune reactions in human filariasis. J Clin Microbiol. 1978; 8(2):228-32. PMC: 275191. DOI: 10.1128/jcm.8.2.228-232.1978. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

McLean B, Sonza S, HOLMES I . Measurement of immunoglobulin A, G, and M class rotavirus antibodies in serum and mucosal secretions. J Clin Microbiol. 1980; 12(3):314-9. PMC: 273582. DOI: 10.1128/jcm.12.3.314-319.1980. View

Hussain R, Hamilton R, Kumaraswami V, Adkinson Jr N, Ottesen E . IgE responses in human filariasis. I. Quantitation of filaria-specific IgE. J Immunol. 1981; 127(4):1623-9. View

Chandrashekar R, Rao U, Subrahmanyam D . Serum dependent cell-mediated immune reactions to Brugia pahangi infective larvae. Parasite Immunol. 1985; 7(6):633-41. DOI: 10.1111/j.1365-3024.1985.tb00107.x. View

Chandrashekar R, Rao U, Rajasekariah G, Subrahmanyam D . Separation of viable microfilariae free of blood cells on Percoll gradients. J Helminthol. 1984; 58(1):69-70. DOI: 10.1017/s0022149x00028078. View

CANLAS M, Wadee A, Lamontagne L, Piessens W . A monoclonal antibody to surface antigens on microfilariae of Brugia malayi reduces microfilaremia in infected jirds. Am J Trop Med Hyg. 1984; 33(3):420-4. DOI: 10.4269/ajtmh.1984.33.420. View

10.

Chandrashekar R, Rao U, Subrahmanyam D, Hopper K, Nelson D, King M . Brugia pahangi: serum-dependent cell-mediated reactions to sheathed and exsheathed microfilariae. Immunology. 1984; 53(3):411-7. PMC: 1454917. View

11.

Aggarwal A, Cuna W, Haque A, Dissous C, Capron A . Resistance against Brugia malayi microfilariae induced by a monoclonal antibody which promotes killing by macrophages and recognizes surface antigen(s). Immunology. 1985; 54(4):655-63. PMC: 1453564. View

12.

Chandrashekar R, Rao U, Parab P, Subrahmanyam D . Brugia malayi: serum dependent cell-mediated reactions to microfilariae. Southeast Asian J Trop Med Public Health. 1985; 16(1):15-21. View

13.

Alkan S, Ball R, Chang J, Braun D . Heterogeneity of cross-reactive idiotypes. Serological and structural analysis of monoclonal anti-p-azobenzene-arsonate antibodies expressing major and minor idiotypes. Mol Immunol. 1983; 20(2):203-11. DOI: 10.1016/0161-5890(83)90132-3. View