The Effect of Hydroalcoholic Extract of Seed on Dehydroepiandrosterone-induced Polycystic Ovarian Syndrome in Rats: An Experimental Study

Overview

Journal Int J Reprod Biomed

Specialty Reproductive Medicine

Date 2021 Apr 12

PMID 33842824

Citations 5

Authors

Samira Khani

Maasoume Abdollahi

Azam Khalaj

Hamid Heidari

Somaye Zohali

Affiliations

Soon will be listed here.

Abstract

Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women.

Objective: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of () seed as a plant, the consumption of which has been recommended in Islam, on dehydroepiandrosterone (DHEA)-induced PCOS rats.

Materials And Methods: This experimental study was carried out on 36 Wistar female rats (3 wk, 60 10 gr). Then rats were divided into 6 groups (n = 6/each): control; PCOS-induced (DHEA 60 mg/kg/sc); PCOS+ Metformine (30 mg/kg); and three experimental groups receiving DHEA + hydroalcoholic extract of seeds in doses of 50, 100 and 200 mg/kg, respectively. Blood samples were taken for the evaluation of sexual hormones, oxidative stress, glucose, and insulin after 30 days of treatment. Ovarian tissue was used for histopathological study.

Results: The serum levels of luteinizing hormone, testosterone, glucose, insulin resistance, malondialdehyde, and insulin (p 0.001) and estrogen increased while the levels of progesterone (p = 0.01) and antioxidant enzymes in the PCOS group decreased (p 0.001).

Conclusion: The administration of the extract to the PCOS rats resulted in remarkable changes in the serumic factors relative to the PCOS group. In addition, the extract improved the structure of the ovarian tissue in the PCOS rat. The histopathological results which are in accordance with biochemical findings imply that seed could be useful in the treatment of PCOS, the higher doses of the extract being more effective.

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