3D Silk Fiber Construct Embedded Dual-Layer PEG Hydrogel for Articular Cartilage Repair - Assessment

Overview

Journal Front Bioeng Biotechnol

Date 2021 Apr 12

PMID 33842448

Citations 6

Authors

Jung Soo Kim

Jaeho Choi

Chang Seok Ki

Ki Hoon Lee

Affiliations

Soon will be listed here.

Abstract

Since articular cartilage does not regenerate itself, researches are underway to heal damaged articular cartilage by applying biomaterials such as a hydrogel. In this study, we have constructed a dual-layer composite hydrogel mimicking the layered structure of articular cartilage. The top layer consists of a high-density PEG hydrogel prepared with 8-arm PEG and PEG diacrylate using thiol-norbornene photo-click chemistry. The compressive modulus of the top layer was 700.1 kPa. The bottom layer consists of a low-density PEG hydrogel reinforced with a 3D silk fiber construct. The low-density PEG hydrogel was prepared with 4-arm PEG using the same cross-linking chemistry, and the compressive modulus was 13.2 kPa. Silk fiber was chosen based on the strong interfacial bonding with the low-density PEG hydrogel. The 3D silk fiber construct was fabricated by moving the silk fiber around the piles using a pile frame, and the compressive modulus of the 3D silk fiber construct was 567 kPa. The two layers were joined through a covalent bond which endowed sufficient stability against repeated torsions. The final 3D silk fiber construct embedded dual-layer PEG hydrogel had a compressive modulus of 744 kPa. Chondrogenic markers confirmed the chondrogenic differentiation of human mesenchymal stem cells encapsulated in the bottom layer.

Citing Articles

Silk fibroin-based hydrogels for cartilage organoids in osteoarthritis treatment.

Shen C, Zhou Z, Li R, Yang S, Zhou D, Zhou F Theranostics. 2025; 15(2):560-584.

PMID: 39744693 PMC: 11671376. DOI: 10.7150/thno.103491.

Hydrogel-Based 3D Bioprinting Technology for Articular Cartilage Regenerative Engineering.

Zhang H, Zhou Z, Zhang F, Wan C Gels. 2024; 10(7).

PMID: 39057453 PMC: 11276275. DOI: 10.3390/gels10070430.

Application of Silk-Fibroin-Based Hydrogels in Tissue Engineering.

Lyu Y, Liu Y, He H, Wang H Gels. 2023; 9(5).

PMID: 37233022 PMC: 10218181. DOI: 10.3390/gels9050431.

Recent Developments and Current Applications of Organic Nanomaterials in Cartilage Repair.

Wei Z, Zhang G, Cao Q, Zhao T, Bian Y, Zhu W Bioengineering (Basel). 2022; 9(8).

PMID: 36004915 PMC: 9405275. DOI: 10.3390/bioengineering9080390.

Natural polymer-based scaffolds for soft tissue repair.

Chen M, Jiang R, Deng N, Zhao X, Li X, Guo C Front Bioeng Biotechnol. 2022; 10:954699.

PMID: 35928962 PMC: 9343850. DOI: 10.3389/fbioe.2022.954699.

References

Vu L, Jain G, Veres B, Rajagopalan P . Cell migration on planar and three-dimensional matrices: a hydrogel-based perspective. Tissue Eng Part B Rev. 2014; 21(1):67-74. PMC: 4321976. DOI: 10.1089/ten.TEB.2013.0782. View

Aigner T, Gebhard P, Schmid E, Bau B, Harley V, Poschl E . SOX9 expression does not correlate with type II collagen expression in adult articular chondrocytes. Matrix Biol. 2003; 22(4):363-72. DOI: 10.1016/s0945-053x(03)00049-0. View

Cheng G, Davoudi Z, Xing X, Yu X, Cheng X, Li Z . Advanced Silk Fibroin Biomaterials for Cartilage Regeneration. ACS Biomater Sci Eng. 2021; 4(8):2704-2715. DOI: 10.1021/acsbiomaterials.8b00150. View

Grande D, Schwartz J, Brandel E, Chahine N, Sgaglione N . Articular Cartilage Repair: Where We Have Been, Where We Are Now, and Where We Are Headed. Cartilage. 2015; 4(4):281-5. PMC: 4297160. DOI: 10.1177/1947603513494402. View

Visser J, Melchels F, Jeon J, van Bussel E, Kimpton L, Byrne H . Reinforcement of hydrogels using three-dimensionally printed microfibres. Nat Commun. 2015; 6:6933. DOI: 10.1038/ncomms7933. View

Agrawal A, Rahbar N, Calvert P . Strong fiber-reinforced hydrogel. Acta Biomater. 2012; 9(2):5313-8. DOI: 10.1016/j.actbio.2012.10.011. View

Zhu J, Marchant R . Design properties of hydrogel tissue-engineering scaffolds. Expert Rev Med Devices. 2011; 8(5):607-26. PMC: 3206299. DOI: 10.1586/erd.11.27. View

Beckett L, Lewis J, Tonge T, Korley L . Enhancement of the Mechanical Properties of Hydrogels with Continuous Fibrous Reinforcement. ACS Biomater Sci Eng. 2020; 6(10):5453-5473. DOI: 10.1021/acsbiomaterials.0c00911. View

Meinel L, Hofmann S, Karageorgiou V, Kirker-Head C, McCool J, Gronowicz G . The inflammatory responses to silk films in vitro and in vivo. Biomaterials. 2004; 26(2):147-55. DOI: 10.1016/j.biomaterials.2004.02.047. View

10.

Rose J, De Laporte L . Hierarchical Design of Tissue Regenerative Constructs. Adv Healthc Mater. 2018; 7(6):e1701067. DOI: 10.1002/adhm.201701067. View

11.

Duan J, Liang X, Zhu K, Guo J, Zhang L . Bilayer hydrogel actuators with tight interfacial adhesion fully constructed from natural polysaccharides. Soft Matter. 2016; 13(2):345-354. DOI: 10.1039/c6sm02089e. View

12.

Kinneberg K, Nelson A, Stender M, Aziz A, Mozdzen L, Harley B . Reinforcement of Mono- and Bi-layer Poly(Ethylene Glycol) Hydrogels with a Fibrous Collagen Scaffold. Ann Biomed Eng. 2015; 43(11):2618-29. PMC: 4618187. DOI: 10.1007/s10439-015-1337-0. View

13.

Little C, Bawolin N, Chen X . Mechanical properties of natural cartilage and tissue-engineered constructs. Tissue Eng Part B Rev. 2011; 17(4):213-27. DOI: 10.1089/ten.TEB.2010.0572. View

14.

Kim J, Kong Y, Niedzielski S, Singh R, Putnam A, Shikanov A . Characterization of the crosslinking kinetics of multi-arm poly(ethylene glycol) hydrogels formed via Michael-type addition. Soft Matter. 2016; 12(7):2076-85. PMC: 4749500. DOI: 10.1039/c5sm02668g. View

15.

Zhan X . Effect of matrix stiffness and adhesion ligand density on chondrogenic differentiation of mesenchymal stem cells. J Biomed Mater Res A. 2019; 108(3):675-683. DOI: 10.1002/jbm.a.36847. View

16.

Zhou W, Feng Y, Yang J, Fan J, Lv J, Zhang L . Electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for endothelial cell growth. J Mater Sci Mater Med. 2015; 26(1):5386. DOI: 10.1007/s10856-015-5386-6. View

17.

Bhattacharjee M, Coburn J, Centola M, Murab S, Barbero A, Kaplan D . Tissue engineering strategies to study cartilage development, degeneration and regeneration. Adv Drug Deliv Rev. 2014; 84:107-22. DOI: 10.1016/j.addr.2014.08.010. View

18.

Kwak H, Ju J, Shin M, Holland C, Lee K . Sericin Promotes Fibroin Silk I Stabilization Across a Phase-Separation. Biomacromolecules. 2017; 18(8):2343-2349. DOI: 10.1021/acs.biomac.7b00549. View

19.

Li X, Sun Q, Li Q, Kawazoe N, Chen G . Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications. Front Chem. 2018; 6:499. PMC: 6204355. DOI: 10.3389/fchem.2018.00499. View

20.

Vega S, Kwon M, Burdick J . Recent advances in hydrogels for cartilage tissue engineering. Eur Cell Mater. 2017; 33:59-75. PMC: 5748291. DOI: 10.22203/eCM.v033a05. View