Bisphenol A and Bisphenol S Oxidative Effects in Sheep Red Blood Cells: An Study

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2021 Apr 9

PMID 33834069

Citations 3

Authors

E Baralla

M P Demontis

M V Varoni

V Pasciu

Affiliations

Soon will be listed here.

Abstract

Bisphenols (BPs) are plastic components widely used worldwide and occurring in the environment. Exposure to these compounds is known to be harmful for animals and humans at different levels. The aim of this study was to evaluate and compare the oxidative effects of bisphenol A (BPA) and bisphenol S (BPS) in sheep. Reactive oxygen species (ROS) production and correlated structural alterations in sheep erythrocytes were investigated . Blood samples from four ewes were collected at fasting from the jugular vein using vacuum collection tubes containing EDTA. For ROS assay in erythrocytes, blood was properly diluted and BPA or BPS was added to obtain final bisphenol concentrations in the range between 1 and 300 M. 2',7'-Dichlorodihydrofluorescein diacetate (H2DCF-DA) 3 M was added to the samples, and fluorescence was read in four replicates using a microplate reader. To evaluate erythrocyte shape, blood smears of blood treated with the different concentrations of BPS and BPA were prepared. A significant increase in ROS production was observed when concentrations of BPS and BPA increased from 1 to 100 M ( < 0.05). At the higher concentrations of the two studied BPs (300 M of BPS and 200-300 M of BPA), a ROS decrease was observed when compared to the control group ( < 0.01). Erythrocytes' shape alterations were observed in cells treated with BPS and BPA 200-300 M 4 hours after the beginning of the treatment. This study confirms that BPA and BPS exhibit oxidative effects on sheep erythrocytes. At higher concentrations, BPA was able to modify erythrocytes' shape, while BPS altered their membrane as a sign of a protein clustering that could lead to eryptosis. These BPs' effects are consequent to intracellular ROS increase.

Citing Articles

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants.

Pasciu V, Nieddu M, Sotgiu F, Baralla E, Berlinguer F Animals (Basel). 2023; 13(14).

PMID: 37508077 PMC: 10376267. DOI: 10.3390/ani13142300.

Natural Products in Mitigation of Bisphenol A Toxicity: Future Therapeutic Use.

Sirasanagandla S, Al-Huseini I, Sakr H, Moqadass M, Das S, Juliana N Molecules. 2022; 27(17).

PMID: 36080155 PMC: 9457803. DOI: 10.3390/molecules27175384.

Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells' Viability In Vitro.

Pasciu V, Sotgiu F, Porcu C, Berlinguer F Animals (Basel). 2021; 11(6).

PMID: 34071487 PMC: 8228453. DOI: 10.3390/ani11061592.

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