Inductively Coupled Plasma Mass Spectrometry Method for Plasma and Intracellular Antimony Quantification Applied to Pharmacokinetics of Meglumine Antimoniate

Overview

Journal Bioanalysis

Specialty Chemistry

Date 2021 Apr 8

PMID 33829863

Citations 1

Authors

Diana J Garay-Baquero

David E Rebellon-Sanchez

Miguel D Prieto

Lina Giraldo-Parra

Adriana Navas

Sheryl Atkinson

Stuart McDougall

Maria Adelaida Gomez

Affiliations

Soon will be listed here.

Abstract

A high-throughput method using inductively coupled plasma mass spectrometry (ICP-MS) was developed and validated for the quantitative analysis of antimony in human plasma and peripheral blood mononuclear cells from patients with cutaneous leishmaniasis undergoing treatment with meglumine antimoniate. Antimony was digested in clinical samples with 1% tetramethylammonium hydroxide/1% EDTA and indium was used as internal standard. Accuracy, precision and stability were evaluated. Taking the lower limit of quantitation to be the lowest validation concentration with precision and accuracy within 20%, the current assay was successfully validated from 25 to 10000 ng/ml for antimony in human plasma and peripheral blood mononuclear cells. This protocol will serve as a baseline for future analytical designs, aiming to provide a reference method to allow inter-study comparisons.

Citing Articles

Cardiovascular Effects of Environmental Metal Antimony: Redox Dyshomeostasis as the Key Pathogenic Driver.

Tan Y, El-Kersh K, Watson S, Wintergerst K, Huang J, Cai L Antioxid Redox Signal. 2022; 38(10-12):803-823.

PMID: 36424825 PMC: 10402706. DOI: 10.1089/ars.2022.0185.

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