Variable and Dose-dependent Response of Saccharomyces and Non-Saccharomyces Yeasts Toward Lignocellulosic Hydrolysate Inhibitors

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2021 Apr 7

PMID 33825150

Authors

Carlos E V F Soares

Jessica C Bergmann

Joao Ricardo Moreira de Almeida

Affiliations

Soon will be listed here.

Abstract

Lignocellulosic hydrolysates will also contain compounds that inhibit microbial metabolism, such as organic acids, furaldehydes, and phenolic compounds. Understanding the response of yeasts toward such inhibitors is important to the development of different bioprocesses. In this work, the growth capacity of 7 industrial Saccharomyces cerevisiae and 7 non-Saccharomyces yeasts was compared in the presence of 3 different concentrations of furaldehydes (furfural and 5-hydroxymetil-furfural), organic acids (acetic and formic acids), and phenolic compounds (vanillin, syringaldehyde, ferulic, and coumaric acids). Then, Candida tropicalis JA2, Meyerozyma caribbica JA9, Wickerhamomyces anomalus 740, S. cerevisiae JP1, B1.1, and G06 were selected for fermentation in presence of acetic acid, HMF, and vanillin because they proved to be most tolerant to the tested compounds, while Spathaspora sp. JA1 because its xylose consumption rate. The results obtained showed a dose-dependent response of the yeasts toward the eight different inhibitors. Among the compared yeasts, S. cerevisiae strains presented higher tolerance than non-Saccharomyces, 3 of them with the highest tolerance among all. Regarding the non-Saccharomyces yeasts, C. tropicalis JA2 and W. anomalus 740 appeared as the most tolerant, whereas Spathaspora strains appeared very sensitive to the different compounds.

References

Alvira P, Tomas-Pejo E, Ballesteros M, Negro M . Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review. Bioresour Technol. 2010; 101(13):4851-61. DOI: 10.1016/j.biortech.2009.11.093. View

Hasunuma T, Kondo A . Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering. Biotechnol Adv. 2011; 30(6):1207-18. DOI: 10.1016/j.biotechadv.2011.10.011. View

Almeida J, Bertilsson M, Gorwa-Grauslund M, Gorsich S, Liden G . Metabolic effects of furaldehydes and impacts on biotechnological processes. Appl Microbiol Biotechnol. 2009; 82(4):625-38. DOI: 10.1007/s00253-009-1875-1. View

Liu Z . Understanding the tolerance of the industrial yeast Saccharomyces cerevisiae against a major class of toxic aldehyde compounds. Appl Microbiol Biotechnol. 2018; 102(13):5369-5390. DOI: 10.1007/s00253-018-8993-6. View

Jonsson L, Martin C . Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects. Bioresour Technol. 2015; 199:103-112. DOI: 10.1016/j.biortech.2015.10.009. View

Kim D, Hong Y, Park H . Co-fermentation of grape must by Issatchenkia orientalis and Saccharomyces cerevisiae reduces the malic acid content in wine. Biotechnol Lett. 2008; 30(9):1633-8. DOI: 10.1007/s10529-008-9726-1. View

Wohlbach D, Kuo A, Sato T, Potts K, Salamov A, LaButti K . Comparative genomics of xylose-fermenting fungi for enhanced biofuel production. Proc Natl Acad Sci U S A. 2011; 108(32):13212-7. PMC: 3156214. DOI: 10.1073/pnas.1103039108. View

Nguyen N, Suh S, Marshall C, Blackwell M . Morphological and ecological similarities: wood-boring beetles associated with novel xylose-fermenting yeasts, Spathaspora passalidarum gen. sp. nov. and Candida jeffriesii sp. nov. Mycol Res. 2006; 110(Pt 10):1232-41. DOI: 10.1016/j.mycres.2006.07.002. View

Sukpipat W, Komeda H, Prasertsan P, Asano Y . Purification and characterization of xylitol dehydrogenase with l-arabitol dehydrogenase activity from the newly isolated pentose-fermenting yeast Meyerozyma caribbica 5XY2. J Biosci Bioeng. 2016; 123(1):20-27. DOI: 10.1016/j.jbiosc.2016.07.011. View

10.

Morais Junior W, Pacheco T, Trichez D, Almeida J, Goncalves S . Xylitol production on sugarcane biomass hydrolysate by newly identified Candida tropicalis JA2 strain. Yeast. 2019; 36(5):349-361. DOI: 10.1002/yea.3394. View

11.

Modig T, Almeida J, Gorwa-Grauslund M, Liden G . Variability of the response of Saccharomyces cerevisiae strains to lignocellulose hydrolysate. Biotechnol Bioeng. 2008; 100(3):423-9. DOI: 10.1002/bit.21789. View

12.

Almeida J, Runquist D, Sanchez I Nogue V, Liden G, Gorwa-Grauslund M . Stress-related challenges in pentose fermentation to ethanol by the yeast Saccharomyces cerevisiae. Biotechnol J. 2011; 6(3):286-99. DOI: 10.1002/biot.201000301. View

13.

Cortez D, Roberto I . Individual and interaction effects of vanillin and syringaldehyde on the xylitol formation by Candida guilliermondii. Bioresour Technol. 2009; 101(6):1858-65. DOI: 10.1016/j.biortech.2009.09.072. View

14.

Almeida J, Karhumaa K, Bengtsson O, Gorwa-Grauslund M . Screening of Saccharomyces cerevisiae strains with respect to anaerobic growth in non-detoxified lignocellulose hydrolysate. Bioresour Technol. 2009; 100(14):3674-7. DOI: 10.1016/j.biortech.2009.02.057. View

15.

Hector R, Mertens J, Bowman M, Nichols N, Cotta M, Hughes S . Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation. Yeast. 2011; 28(9):645-60. DOI: 10.1002/yea.1893. View

16.

Basso L, de Amorim H, de Oliveira A, Lopes M . Yeast selection for fuel ethanol production in Brazil. FEMS Yeast Res. 2008; 8(7):1155-63. DOI: 10.1111/j.1567-1364.2008.00428.x. View

17.

Reis V, Nicola A, de Souza Oliveira Neto O, Batista V, Moraes L, Torres F . Genetic characterization and construction of an auxotrophic strain of Saccharomyces cerevisiae JP1, a Brazilian industrial yeast strain for bioethanol production. J Ind Microbiol Biotechnol. 2012; 39(11):1673-83. DOI: 10.1007/s10295-012-1170-5. View

18.

Shen Y, Li H, Wang X, Zhang X, Hou J, Wang L . High vanillin tolerance of an evolved Saccharomyces cerevisiae strain owing to its enhanced vanillin reduction and antioxidative capacity. J Ind Microbiol Biotechnol. 2014; 41(11):1637-45. DOI: 10.1007/s10295-014-1515-3. View

19.

Mota T, de Souza W, Oliveira D, Martins P, Sampaio B, Vinecky F . Suppression of a BAHD acyltransferase decreases p-coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis. Plant J. 2020; 105(1):136-150. DOI: 10.1111/tpj.15046. View

20.

Su Y, Willis L, Jeffries T . Effects of aeration on growth, ethanol and polyol accumulation by Spathaspora passalidarum NRRL Y-27907 and Scheffersomyces stipitis NRRL Y-7124. Biotechnol Bioeng. 2014; 112(3):457-69. DOI: 10.1002/bit.25445. View