Temporal Oral Microbiome Changes with Brushing in Children with Cleft Lip and Palate

Overview

Journal Heliyon

Specialty Social Sciences

Date 2021 Apr 5

PMID 33817376

Citations 4

Authors

Rita Rodrigues

Ana P Chung

Martin S Mortensen

Maria H Fernandes

Antonio B Monteiro

Rowney Furfuro

Catia C Silva

Maria C Manso

Soren J Sorensen

Paula V Morais

Affiliations

Soon will be listed here.

Abstract

This cohort study aimed to characterize the oral microbiome of children with CLP, from two different age groups, and evaluate the effect of supervised or unsupervised toothbrushing on the microbiome of the cleft over time. Swab samples were collected from the cleft area at three different time points (A; no brushing, B; after 15 days and C; after 30 days) and were analyzed using next-generation sequencing to determine the microbial composition and diversity in these time points. Overall, brushing significantly decreased the abundance of the genera and in the two age groups examined, and for this decrease was more evident for children (2-6 years old). In the preteen group (7-12 years old), a significant relative increase of the genus was observed after brushing. In this study, the systematic brushing over a period of thirty days also resulted in differences at the intra-individual bacterial richness.

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