The Isolation and Replication of African Swine Fever Virus in Primary Renal-Derived Swine Macrophages

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2021 Apr 5

PMID 33816588

Citations 9

Authors

Taehwan Oh

Duy Tien Do

Hung Van Vo

Hyeok-Il Kwon

Seung-Chul Lee

Min Ho Kim

Dung Thi Thu Nguyen

Quang Tin Vinh Le

Tan Minh Tran

Toan Tat Nguyen

Joo Young Lee

Chanhee Chae

Affiliations

Soon will be listed here.

Abstract

African swine fever virus (ASFV) causes hemorrhagic disease in domestic pigs by replicating mainly in monocyte/macrophage lineages. Various primary cells including pulmonary alveolar macrophages have been used for the propagation of ASFV on this account. However, ethical constraints and consistency problems exist as it is necessary to harvest same phenotype of primary cells in order to continue a study. We suggested renal-derived swine macrophages as a novel primary cell candidate to address these issues. These primary cells proved to be permissive to both cell adapted ASFV and a wild-type ASFV. Compared to the commercial cell line MA-104, the renal-derived macrophages were more suitable to isolate the field virus. The consistent molecular characteristics of the renal-derived macrophages were demonstrated by immunocytochemistry with antibodies against macrophage cell surface markers including CD163, CD172a, and Iba-1. Viral protein p30 and p72 expression in ASFV infected macrophages was confirmed by immunocytochemistry by use of specific monoclonal antibodies. We observed increase of cell-free viral DNA and infectious virus titer in infected cell supernatant in successive days-post-infection. These results demonstrated that primary renal-derived swine macrophages are useful for ASFV isolation and propagation in terms of cell phenotypes, susceptibility to the virus, and virus production.

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