Development of a Point-of-Care Assay for HIV-1 Viral Load Using Higher Refractive Index Antibody-Coated Microbeads

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2021 Apr 3

PMID 33807789

Citations 5

Authors

Mazhar Sher

Benjamin Coleman

Massimo Caputi

Waseem Asghar

Affiliations

Soon will be listed here.

Abstract

The detection of viruses using imaging techniques is challenging because of the weak scattering of light generated by the targets of sizes in the nanometer range. The system we have developed overcomes the light scattering problems by utilizing antibody-coated microbeads of higher index of refraction that can specifically bind with viruses and increase the acceptance angle. Using the new technology, we have developed a portable, cost-effective, and field-deployable platform for the rapid quantification of HIV-1 viral load for point-of-care (POC) settings. The system combines microfluidics with a wide field of view lensless imaging technology. Highly specific antibodies are functionalized to a glass slide inside a microchip to capture HIV-1 virions. The captured virions are then bound by antibody-conjugated microbeads, which have a higher refraction index. The microbeads-HIV-1 virions complexes generate diffraction patterns that are detected with a custom-built imaging setup and rapidly and accurately quantified by computational analysis. This platform technology enables fast nanoscale virus imaging and quantification from biological samples and thus can play a significant role in the detection and management of viral diseases.

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References

Roingeard P . Viral detection by electron microscopy: past, present and future. Biol Cell. 2008; 100(8):491-501. PMC: 7161876. DOI: 10.1042/BC20070173. View

Asghar W, Sher M, Khan N, Vyas J, Demirci U . Microfluidic Chip for Detection of Fungal Infections. ACS Omega. 2019; 4(4):7474-7481. PMC: 6504191. DOI: 10.1021/acsomega.9b00499. View

Shafiee H, Wang S, Inci F, Toy M, Henrich T, Kuritzkes D . Emerging technologies for point-of-care management of HIV infection. Annu Rev Med. 2014; 66:387-405. DOI: 10.1146/annurev-med-092112-143017. View

Quinn T . Acute primary HIV infection. JAMA. 1997; 278(1):58-62. View

Emamzadeh-Fard S, Fard S, SeyedAlinaghi S, Paydary K . Adherence to anti-retroviral therapy and its determinants in HIV/AIDS patients: a review. Infect Disord Drug Targets. 2012; 12(5):346-56. DOI: 10.2174/187152612804142251. View

Broder S . The development of antiretroviral therapy and its impact on the HIV-1/AIDS pandemic. Antiviral Res. 2009; 85(1):1-18. PMC: 2815149. DOI: 10.1016/j.antiviral.2009.10.002. View

Kowalski M, Potz J, Basiripour L, Dorfman T, Goh W, Terwilliger E . Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1. Science. 1987; 237(4820):1351-5. DOI: 10.1126/science.3629244. View

Ray A, Khalid M, Demcenko A, Daloglu M, Tseng D, Reboud J . Holographic detection of nanoparticles using acoustically actuated nanolenses. Nat Commun. 2020; 11(1):171. PMC: 6965092. DOI: 10.1038/s41467-019-13802-1. View

DeMarino C, Pleet M, Cowen M, Barclay R, Akpamagbo Y, Erickson J . Antiretroviral Drugs Alter the Content of Extracellular Vesicles from HIV-1-Infected Cells. Sci Rep. 2018; 8(1):7653. PMC: 5955991. DOI: 10.1038/s41598-018-25943-2. View

10.

Briggs J, Wilk T, Welker R, Krausslich H, Fuller S . Structural organization of authentic, mature HIV-1 virions and cores. EMBO J. 2003; 22(7):1707-15. PMC: 152888. DOI: 10.1093/emboj/cdg143. View

11.

Kim Y, Moon S, Kuritzkes D, Demirci U . Quantum dot-based HIV capture and imaging in a microfluidic channel. Biosens Bioelectron. 2009; 25(1):253-8. PMC: 2746625. DOI: 10.1016/j.bios.2009.06.023. View

12.

Ilyas S, Sher M, Du E, Asghar W . Smartphone-based sickle cell disease detection and monitoring for point-of-care settings. Biosens Bioelectron. 2020; 165:112417. PMC: 7484220. DOI: 10.1016/j.bios.2020.112417. View

13.

Goldsmith C, Miller S . Modern uses of electron microscopy for detection of viruses. Clin Microbiol Rev. 2009; 22(4):552-63. PMC: 2772359. DOI: 10.1128/CMR.00027-09. View

14.

Daaboul G, Freedman D, Scherr S, Carter E, Rosca A, Bernstein D . Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses. PLoS One. 2017; 12(6):e0179728. PMC: 5484481. DOI: 10.1371/journal.pone.0179728. View

15.

Pope M, Haase A . Transmission, acute HIV-1 infection and the quest for strategies to prevent infection. Nat Med. 2003; 9(7):847-52. DOI: 10.1038/nm0703-847. View

16.

Fennell R, Sher M, Asghar W . Design, Development, and Performance Comparison of Wide Field Lensless and Lens-Based Optical Systems for Point-of-Care Biological Applications. Opt Lasers Eng. 2020; 137. PMC: 7472995. DOI: 10.1016/j.optlaseng.2020.106326. View

17.

Ganesh P, Heller T, Chione B, Gumulira J, Gugsa S, Khan S . Near Point-of-Care HIV Viral Load: Targeted Testing at Large Facilities. J Acquir Immune Defic Syndr. 2020; 86(2):258-263. PMC: 7803448. DOI: 10.1097/QAI.0000000000002555. View

18.

Daaboul G, Yurt A, Zhang X, Hwang G, Goldberg B, Unlu M . High-throughput detection and sizing of individual low-index nanoparticles and viruses for pathogen identification. Nano Lett. 2010; 10(11):4727-31. DOI: 10.1021/nl103210p. View

19.

Gorocs Z, Ozcan A . On-chip biomedical imaging. IEEE Rev Biomed Eng. 2013; 6:29-46. PMC: 3621021. DOI: 10.1109/RBME.2012.2215847. View

20.

Coarsey C, Coleman B, Kabir M, Sher M, Asghar W . Development of a Flow-Free Magnetic Actuation Platform for an Automated Microfluidic ELISA. RSC Adv. 2019; 9(15):8159-8168. PMC: 6880949. DOI: 10.1039/C8RA07607C. View