Atractylodin Produces Antinociceptive Effect Through a Long-Lasting TRPA1 Channel Activation

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Apr 3

PMID 33807167

Citations 4

Authors

Hirosato Kanda

Yanjing Yang

Shaoqi Duan

Yoko Kogure

Shenglan Wang

Emiko Iwaoka

Miku Ishikawa

Saki Takeda

Hidemi Sonoda

Kyoka Mizuta

Shunji Aoki

Satoshi Yamamoto

Koichi Noguchi

Yi Dai

Affiliations

Soon will be listed here.

Abstract

Atractylodin (ATR) is a bioactive component found in dried rhizomes of (AL) De Candolle. Although AL has accumulated empirical evidence for the treatment of pain, the molecular mechanism underlying the anti-pain effect of ATR remains unclear. In this study, we found that ATR increases transient receptor potential ankyrin-1 (TRPA1) single-channel activity in hTRPA1 expressing HEK293 cells. A bath application of ATR produced a long-lasting calcium response, and the response was completely diminished in the dorsal root ganglion neurons of TRPA1 knockout mice. Intraplantar injection of ATR evoked moderate and prolonged nociceptive behavior compared to the injection of allyl isothiocyanate (AITC). Systemic application of ATR inhibited AITC-induced nociceptive responses in a dose-dependent manner. Co-application of ATR and QX-314 increased the noxious heat threshold compared with AITC in vivo. Collectively, we concluded that ATR is a unique agonist of TRPA1 channels, which produces long-lasting channel activation. Our results indicated ATR-mediated anti-nociceptive effect through the desensitization of TRPA1-expressing nociceptors.

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