Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Apr 3

PMID 33804943

Citations 6

Authors

Tomasz Ligor

Joanna Zawadzka

Grzegorz Straczynski

Rosa M Gonzalez Paredes

Anna Wenda-Piesik

Ileana Andreea Ratiu

Marek Muszytowski

Affiliations

Soon will be listed here.

Abstract

Volatile organic compounds (VOCs) exiting in urine are potential biomarkers of chronic kidney diseases. Headspace solid phase microextraction (HS-SPME) was applied for extraction VOCs over the urine samples. Volatile metabolites were separated and identified by means of two-dimensional gas chromatography and time of flight mass spectrometry (GC × GC TOF MS). Patients with glomerular diseases ( = 27) and healthy controls ( = 20) were recruited in the study. Different VOCs profiles were obtained from patients and control. Developed methodology offers the opportunity to examine the metabolic profile associated with glomerulopathy. Four compounds found in elevated amounts in the patients group, i.e., methyl hexadecanoate; 9-hexadecen-1-ol; 6,10-dimethyl-5,9-undecadien-2-one and 2-pentanone were proposed as markers of glomerular diseases.

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