The Effect of Cultivation Passaging on the Relative Telomere Length and Proliferation Capacity of Dental Pulp Stem Cells

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2021 Apr 3

PMID 33804786

Citations 6

Authors

Nela Pilbauerova

Tomas Soukup

Tereza Suchankova Kleplova

Jan Schmidt

Jakub Suchanek

Affiliations

Soon will be listed here.

Abstract

Telomeres are repetitive nucleoprotein DNA sequences that shorten with each cell division. The stem cells activate telomerase to compensate for the telomere loss. This study aimed to evaluate the effect of cultivation passaging on the relative telomere length and proliferation capacity of dental pulp stem cells. We used ten dental pulp stem cell (DPSC) lineages stored for 12 months using uncontrolled-rate freezing to reach the study's goal. We analyzed their proliferation rate, phenotype using flow cytometry, multipotency, and relative telomere length using a qPCR analysis. We determined the relative telomere length in the added study by performing analysis after one, two, and three weeks of cultivation with no passaging. We documented the telomere attrition with increasing passaging. The shorter the relative telomere length, the lower reached population doublings, and longer population doubling time were observed at the end of the cultivation. We observed the telomere prolongation in DPSCs cultivated for two weeks with no passaging in the added subsequent study. We concluded that excessive proliferation demands on DPSCs during in vitro cultivation result in telomere attrition. We opened the theory that the telomerase might be more efficient during cell cultivation with no passaging. This observation could help in preserving the telomere length during ex vivo DPSC expansion.

Citing Articles

Intrinsic and extrinsic modulators of human dental pulp stem cells: advancing strategies for tissue engineering applications.

Kavakebian F, Rezapour A, Seyedebrahimi R, Farsani M, Fakhr M, Zare Jalise S Mol Biol Rep. 2025; 52(1):190.

PMID: 39899148 DOI: 10.1007/s11033-025-10281-0.

Effect of Human Platelet Lysate as Cultivation Nutrient Supplement on Human Natal Dental Pulp Stem Cell In Vitro Expansion.

Pilbauerova N, Schmidt J, Suchankova Kleplova T, Soukup T, Suchanek J Biomolecules. 2022; 12(8).

PMID: 36008985 PMC: 9405745. DOI: 10.3390/biom12081091.

Intra-Individual Variability of Human Dental Pulp Stem Cell Features Isolated from the Same Donor.

Pilbauerova N, Schmidt J, Soukup T, Duska J, Suchanek J Int J Mol Sci. 2021; 22(24).

PMID: 34948330 PMC: 8709021. DOI: 10.3390/ijms222413515.

Tissue Regeneration and Physiological Functional Recovery in Dental and Craniofacial Fields.

Ouchi T, Nakagawa T Biomolecules. 2021; 11(11).

PMID: 34827642 PMC: 8615394. DOI: 10.3390/biom11111644.

Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells.

Gil-Recio C, Montori S, Al Demour S, Ababneh M, Ferres-Padro E, Marti C Stem Cells Int. 2021; 2021:5212852.

PMID: 34795766 PMC: 8593589. DOI: 10.1155/2021/5212852.

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