Complementary Analytical Platforms of NMR Spectroscopy and LCMS Analysis in the Metabolite Profiling of

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2021 Apr 3

PMID 33801258

Citations 8

Authors

Muhammad Safwan Ahamad Bustamam

Hamza Ahmed Pantami

Awanis Azizan

Khozirah Shaari

Chong Chou Min

Faridah Abas

Norio Nagao

Maulidiani Maulidiani

Sanjoy Banerjee

Fadzil Sulaiman

Intan Safinar Ismail

Affiliations

Soon will be listed here.

Abstract

This study was designed to profile the metabolites of , an indigenous and less explored microalgae species. H Nuclear Magnetic Resonance (NMR) spectroscopy and Liquid Chromatography-Mass Spectrometry (LCMS) were used to establish the metabolite profiles of five different extracts of this microalga, which are hexane (Hex), ethyl acetate (EtOAc), absolute ethanol (EtOH), EtOH:water 1:1 (AqE), and 100% water (Aq). Partial least square discriminant analysis (PLS-DA) of the generated profiles revealed that EtOAc and Aq extracts contain a diverse range of metabolites as compared to the other extracts with a total of twenty-one metabolites, comprising carotenoids, polyunsaturated fatty acids, and amino acids, that were putatively identified from the NMR spectra. Meanwhile, thirty-two metabolites were successfully annotated from the LCMS/MS data, ten of which (palmitic acid, oleic acid, α-linolenic acid, arachidic acid, cholesterol, DHA, DPA, fucoxanthin, astaxanthin, and pheophytin) were similar to those present in the NMR profile. Another eleven glycerophospholipids were discovered using MS/MS-based molecular network (MN) platform. The results of this study, besides providing a better understanding of 's chemical make-up, will be of importance in exploring this species potential as a feed ingredient in the aquaculture industry.

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References

Azizan A, Ahamad Bustamam M, Maulidiani M, Shaari K, Safinar Ismail I, Nagao N . Metabolite Profiling of the Microalgal Diatom and Correlation with Antioxidant and Nitric Oxide Inhibitory Activities via ¹H NMR-Based Metabolomics. Mar Drugs. 2018; 16(5). PMC: 5983285. DOI: 10.3390/md16050154. View

Galal A, Reda R, Mohamed A . Influences of Chlorella vulgaris dietary supplementation on growth performance, hematology, immune response and disease resistance in Oreochromis niloticus exposed to sub-lethal concentrations of penoxsulam herbicide. Fish Shellfish Immunol. 2018; 77:445-456. DOI: 10.1016/j.fsi.2018.04.011. View

Buedenbender L, Astone F, Tasdemir D . Bioactive Molecular Networking for Mapping the Antimicrobial Constituents of the Baltic Brown Alga . Mar Drugs. 2020; 18(6). PMC: 7345172. DOI: 10.3390/md18060311. View

Lv M, Lo C, Hsu C, Wang Y, Chiang Y, Sun Q . Identification of Enantiomeric Byproducts During Microalgae-Mediated Transformation of Metoprolol by MS/MS Spectrum Based Networking. Front Microbiol. 2018; 9:2115. PMC: 6137207. DOI: 10.3389/fmicb.2018.02115. View

Nedbalova L, Strizek A, Sigler K, rezanka T . Effect of salinity on the fatty acid and triacylglycerol composition of five haptophyte algae from the genera Coccolithophora, Isochrysis and Prymnesium determined by LC-MS/APCI. Phytochemistry. 2016; 130:64-76. DOI: 10.1016/j.phytochem.2016.06.001. View

Ghioni C, Porter A, Sadler I, Tocher D, Sargent J . Cultured fish cells metabolize octadecapentaenoic acid (all-cis delta3,6,9,12,15-18:5) to octadecatetraenoic acid (all-cis delta6,9,12,15-18:4) via its 2-trans intermediate (trans delta2, all-cis delta6,9,12,15-18:5). Lipids. 2001; 36(2):145-52. DOI: 10.1007/s11745-001-0701-0. View

Ibanez C, Simo C, Barupal D, Fiehn O, Kivipelto M, Cedazo-Minguez A . A new metabolomic workflow for early detection of Alzheimer's disease. J Chromatogr A. 2013; 1302:65-71. DOI: 10.1016/j.chroma.2013.06.005. View

Kim S, Jung Y, Kwon O, Cha K, Um B, Chung D . A potential commercial source of fucoxanthin extracted from the microalga Phaeodactylum tricornutum. Appl Biochem Biotechnol. 2012; 166(7):1843-55. DOI: 10.1007/s12010-012-9602-2. View

Goodacre R, Vaidyanathan S, Dunn W, Harrigan G, Kell D . Metabolomics by numbers: acquiring and understanding global metabolite data. Trends Biotechnol. 2004; 22(5):245-52. DOI: 10.1016/j.tibtech.2004.03.007. View

10.

Bingol K, Bruschweiler-Li L, Li D, Zhang B, Xie M, Bruschweiler R . Emerging new strategies for successful metabolite identification in metabolomics. Bioanalysis. 2016; 8(6):557-73. PMC: 5493966. DOI: 10.4155/bio-2015-0004. View

11.

Meireles L, Guedes A, Malcata F . Lipid class composition of the microalga Pavlova lutheri: eicosapentaenoic and docosahexaenoic acids. J Agric Food Chem. 2003; 51(8):2237-41. DOI: 10.1021/jf025952y. View

12.

Waters N, Holmes E, Williams A, Waterfield C, Farrant R, Nicholson J . NMR and pattern recognition studies on the time-related metabolic effects of alpha-naphthylisothiocyanate on liver, urine, and plasma in the rat: an integrative metabonomic approach. Chem Res Toxicol. 2001; 14(10):1401-12. DOI: 10.1021/tx010067f. View

13.

Pantami H, Ahamad Bustamam M, Lee S, Safinar Ismail I, Mohd Faudzi S, Nakakuni M . Comprehensive GCMS and LC-MS/MS Metabolite Profiling of . Mar Drugs. 2020; 18(7). PMC: 7404257. DOI: 10.3390/md18070367. View

14.

Goh Jr L, Loh S, Fatimah M, Perumal K . Bioaccessibility of Carotenoids and Tocopherols in Marine Microalgae, Nannochloropsis sp. and Chaetoceros sp. Malays J Nutr. 2012; 15(1):77-86. View

15.

Ahmed F, Fanning K, Netzel M, Turner W, Li Y, Schenk P . Profiling of carotenoids and antioxidant capacity of microalgae from subtropical coastal and brackish waters. Food Chem. 2014; 165:300-6. DOI: 10.1016/j.foodchem.2014.05.107. View

16.

Farag M, Huhman D, Lei Z, Sumner L . Metabolic profiling and systematic identification of flavonoids and isoflavonoids in roots and cell suspension cultures of Medicago truncatula using HPLC-UV-ESI-MS and GC-MS. Phytochemistry. 2006; 68(3):342-54. DOI: 10.1016/j.phytochem.2006.10.023. View

17.

Pulz O, Gross W . Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol. 2004; 65(6):635-48. DOI: 10.1007/s00253-004-1647-x. View

18.

Nguyen D, Wu C, Moree W, Lamsa A, Medema M, Zhao X . MS/MS networking guided analysis of molecule and gene cluster families. Proc Natl Acad Sci U S A. 2013; 110(28):E2611-20. PMC: 3710860. DOI: 10.1073/pnas.1303471110. View

19.

Farag M, Wessjohann L . Metabolome classification of commercial Hypericum perforatum (St. John's Wort) preparations via UPLC-qTOF-MS and chemometrics. Planta Med. 2012; 78(5):488-96. DOI: 10.1055/s-0031-1298170. View

20.

Van Der Kooy F, Maltese F, Choi Y, Kim H, Verpoorte R . Quality control of herbal material and phytopharmaceuticals with MS and NMR based metabolic fingerprinting. Planta Med. 2009; 75(7):763-75. DOI: 10.1055/s-0029-1185450. View