Strategies for Cryopreservation of Testicular Cells and Tissues in Cancer and Genetic Diseases

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 2021 Apr 1

PMID 33791878

Citations 9

Authors

Tanushree Patra

Devendra Pathak

Mukesh Kumar Gupta

Affiliations

Soon will be listed here.

Abstract

Cryopreservation of testicular cells and tissues is useful for the preservation and restoration of fertility in pre-pubertal males expecting gonadotoxic treatment for cancer and genetic diseases causing impaired spermatogenesis. A number of freezing and vitrification protocols have thus been tried and variable results have been reported in terms of cell viability spermatogenesis progression and the production of fertile spermatozoa. A few studies have also reported the production of live offspring from cryopreserved testicular stem cells and tissues in rodents but their replication in large animals and human have been lacking. Advancement in in vitro spermatogenesis system has improved the possibility of producing fertile spermatozoa from the cryopreserved testis and has reduced the dependency on transplantation. This review provides an update on various cryopreservation strategies for fertility preservation in males expecting gonadotoxic treatment. It also discusses various methods of assessing and ameliorating cryoinjuries. Newer developments on in vitro spermatogenesis and testicular tissue engineering for in vitro sperm production from cryopreserved SSCs and testicular tissue are also discussed.

Citing Articles

Transcriptomic Differences by RNA Sequencing for Evaluation of New Method for Long-Time In Vitro Culture of Cryopreserved Testicular Tissue for Oncologic Patients.

Pei C, Todorov P, Kong Q, Cao M, Isachenko E, Rahimi G Cells. 2024; 13(18.

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A century of andrology in Cell & Tissue Research: looking back while moving forward.

Meinhardt A, Sutovsky P Cell Tissue Res. 2024; .

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Bridging the Gap: Animal Models in Next-Generation Reproductive Technologies for Male Fertility Preservation.

Aponte P, Gutierrez-Reinoso M, Garcia-Herreros M Life (Basel). 2024; 14(1).

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Comparative Transcriptomic Analyses for the Optimization of Thawing Regimes during Conventional Cryopreservation of Mature and Immature Human Testicular Tissue.

Pei C, Todorov P, Cao M, Kong Q, Isachenko E, Rahimi G Int J Mol Sci. 2024; 25(1).

PMID: 38203385 PMC: 10778995. DOI: 10.3390/ijms25010214.

New Solutions for Old Problems: How Reproductive Tissue Engineering Has Been Revolutionizing Reproductive Medicine.

Leonel E, Dadashzadeh A, Moghassemi S, Vlieghe H, Wyns C, Orellana R Ann Biomed Eng. 2023; 51(10):2143-2171.

PMID: 37468688 DOI: 10.1007/s10439-023-03321-y.

References

Abrishami M, Abbasi S, Honaramooz A . The effect of donor age on progression of spermatogenesis in canine testicular tissue after xenografting into immunodeficient mice. Theriogenology. 2009; 73(4):512-22. DOI: 10.1016/j.theriogenology.2009.09.035. View

Abrishami M, Anzar M, Yang Y, Honaramooz A . Cryopreservation of immature porcine testis tissue to maintain its developmental potential after xenografting into recipient mice. Theriogenology. 2009; 73(1):86-96. DOI: 10.1016/j.theriogenology.2009.08.004. View

Arav A, Natan Y, Kalo D, Komsky-Elbaz A, Roth Z, Levi-Setti P . A new, simple, automatic vitrification device: preliminary results with murine and bovine oocytes and embryos. J Assist Reprod Genet. 2018; 35(7):1161-1168. PMC: 6063841. DOI: 10.1007/s10815-018-1210-9. View

Arkoun B, Dumont L, Milazzo J, Rondanino C, Bironneau A, Wils J . Does soaking temperature during controlled slow freezing of pre-pubertal mouse testes influence course of in vitro spermatogenesis?. Cell Tissue Res. 2015; 364(3):661-674. DOI: 10.1007/s00441-015-2341-2. View

Arkoun B, Dumont L, Milazzo J, Way A, Bironneau A, Wils J . Retinol improves in vitro differentiation of pre-pubertal mouse spermatogonial stem cells into sperm during the first wave of spermatogenesis. PLoS One. 2015; 10(2):e0116660. PMC: 4340963. DOI: 10.1371/journal.pone.0116660. View

Baert Y, Dvorakova-Hortova K, Margaryan H, Goossens E . Mouse in vitro spermatogenesis on alginate-based 3D bioprinted scaffolds. Biofabrication. 2019; 11(3):035011. DOI: 10.1088/1758-5090/ab1452. View

Baert Y, Goossens E . Preparation of Scaffolds from Decellularized Testicular Matrix. Methods Mol Biol. 2017; 1577:121-127. DOI: 10.1007/7651_2017_29. View

Baert Y, Onofre J, Van Saen D, Goossens E . Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing. Methods Mol Biol. 2018; 1748:287-294. DOI: 10.1007/978-1-4939-7698-0_20. View

Baert Y, Van Saen D, Haentjens P, Int Veld P, Tournaye H, Goossens E . What is the best cryopreservation protocol for human testicular tissue banking?. Hum Reprod. 2013; 28(7):1816-26. DOI: 10.1093/humrep/det100. View

10.

Balduzzi A, Dalle J, Jahnukainen K, von Wolff M, Lucchini G, Ifversen M . Fertility preservation issues in pediatric hematopoietic stem cell transplantation: practical approaches from the consensus of the Pediatric Diseases Working Party of the EBMT and the International BFM Study Group. Bone Marrow Transplant. 2017; 52(10):1406-1415. DOI: 10.1038/bmt.2017.147. View

11.

Bebbere D, Pinna S, Nieddu S, Natan D, Arav A, Ledda S . Gene expression analysis of ovine prepubertal testicular tissue vitrified with a novel cryodevice (E.Vit). J Assist Reprod Genet. 2019; 36(10):2145-2154. PMC: 6823412. DOI: 10.1007/s10815-019-01559-x. View

12.

Bielanski A, Nadin-Davis S, Sapp T . Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology. 2000; 40(2):110-6. DOI: 10.1006/cryo.1999.2227. View

13.

Braye A, Tournaye H, Goossens E . Setting Up a Cryopreservation Programme for Immature Testicular Tissue: Lessons Learned After More Than 15 Years of Experience. Clin Med Insights Reprod Health. 2019; 13:1179558119886342. PMC: 6868573. DOI: 10.1177/1179558119886342. View

14.

Chatdarong K, Thuwanut P, Morrell J . The development of cat testicular sperm cryopreservation protocols: Effects of tissue fragments or sperm cell suspension. Theriogenology. 2015; 85(2):200-6. DOI: 10.1016/j.theriogenology.2015.09.030. View

15.

Corkum K, Lautz T, Johnson E, Reimann M, Walz A, Lockart B . Testicular wedge biopsy for fertility preservation in children at significant risk for azoospermia after gonadotoxic therapy. J Pediatr Surg. 2019; 54(9):1901-1905. DOI: 10.1016/j.jpedsurg.2019.01.055. View

16.

Costa G, Avelar G, Lacerda S, Figueiredo A, Tavares A, Rezende-Neto J . Horse spermatogonial stem cell cryopreservation: feasible protocols and potential biotechnological applications. Cell Tissue Res. 2017; 370(3):489-500. DOI: 10.1007/s00441-017-2673-1. View

17.

da Silva A, Bezerra L, Praxedes E, Moreira S, de Souza C, Oliveira M . Combination of intracellular cryoprotectants preserves the structure and the cells proliferative capacity potential of adult collared peccary testicular tissue subjected to solid surface vitrification. Cryobiology. 2019; 91:53-60. DOI: 10.1016/j.cryobiol.2019.10.199. View

18.

de Michele F, Poels J, Weerens L, Petit C, Evrard Z, Ambroise J . Preserved seminiferous tubule integrity with spermatogonial survival and induction of Sertoli and Leydig cell maturation after long-term organotypic culture of prepubertal human testicular tissue. Hum Reprod. 2016; 32(1):32-45. DOI: 10.1093/humrep/dew300. View

19.

Devi L, Makala H, Pothana L, Nirmalkar K, Goel S . Comparative efficacies of six different media for cryopreservation of immature buffalo (Bubalus bubalis) calf testis. Reprod Fertil Dev. 2014; 28(7):872-885. DOI: 10.1071/RD14171. View

20.

Dinnyes A, Dai Y, Jiang S, Yang X . High developmental rates of vitrified bovine oocytes following parthenogenetic activation, in vitro fertilization, and somatic cell nuclear transfer. Biol Reprod. 2000; 63(2):513-8. DOI: 10.1095/biolreprod63.2.513. View