Analysis of in Vitro and in Vivo Products of the TMV 30kDa Open Reading Frame Using Antisera Raised Against a Synthetic Peptide

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2021 Mar 31

PMID 33785967

Authors

Paula A Kiberstis

Antonello Pessi

Eric Atherton

Richard Jackson

Tony Hunter

David Zimmern

Affiliations

Soon will be listed here.

Abstract

The peptide Tyr-Ser-Glu-Ala-Thr-Val-Ala-Glu-Ser-Asp-Ser-Phe (the predicted C-terminal 11 amino acids of the TMV 30kDa open reading frame plus an additional N-terminal Tyr residue) was synthesized by solid phase methods and used to raise antisera in rabbits. These antisera precipitated 4 major proteins (p30, p28, p19 and 18.5kDa) from in vitro translation products of TMV short rod RNA, but only one, of apparent = 30500, from TMV-infected tobacco protoplasts. This protein was made between 8 and 16 h post infection, and had [S]Met-labelled tryptic peptides identical to those of in vitro synthesized p30.

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