3,5-Dimethoxy-4-benzoic Acid (syringic Acid) a Natural Phenolic Acid Reduces Reactive Oxygen Species in Differentiated 3T3-L1 Adipocytes

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 2021 Mar 27

PMID 33772407

Citations 5

Authors

Cordelia Mano John

Sumathy Arockiasamy

Affiliations

Soon will be listed here.

Abstract

Preadipocytes under nutrient excess mature to lipid-laden adipocytes that are hotspots for generation of reactive oxygen species (ROS) imbalance and oxidative stress. Syringic acid (SA), a natural phenolic acid, was evaluated for its in vitro antioxidant and ROS modulation during in matured 3T3-L1 adipocytes. Following 10 d, the SA-treated adipocytes were evaluated for the levels of glutathione (GSH) and antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT). The levels of peroxides in mature adipocytes were estimated using dichlorofluorescein (DCF) cleavage fluorescence. The level of NADPH oxidase 4 (NOX4) expression was also investigated following 10-d differentiation period. SA significantly improved the levels of GSH, SOD, and CAT in matured adipocytes. Reduction in ROS production levels was also witnessed by decrease in DCF cleavage. SA showed concentration-dependent inhibition of NOX4 by day 7 of adipogenesis when compared with differentiated and undifferentiated cells. Moreover, SA exhibited effective antioxidant and anti-radical scavenging activity. These results suggest that SA in addition to inhibiting adipogenesis can strongly reduce ROS stress in mature adipocytes by upregulating levels of intracellular antioxidants and decreasing levels of NOX4 in 3T3-L1 adipocytes.

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References

Adachi T, Toishi T, Wu H, Kamiya T, Hara H . Expression of extracellular superoxide dismutase during adipose differentiation in 3T3-L1 cells. Redox Rep. 2009; 14(1):34-40. DOI: 10.1179/135100009X392467. View

Ajitha M, Mohanlal S, Suresh C, Jayalekshmy A . DPPH radical scavenging activity of tricin and its conjugates isolated from "Njavara" rice bran: a density functional theory study. J Agric Food Chem. 2012; 60(14):3693-9. DOI: 10.1021/jf204826e. View

Al-Farsi M, Alasalvar C, Morris A, Baron M, Shahidi F . Comparison of antioxidant activity, anthocyanins, carotenoids, and phenolics of three native fresh and sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. J Agric Food Chem. 2005; 53(19):7592-9. DOI: 10.1021/jf050579q. View

Araki S, Dobashi K, Kubo K, Yamamoto Y, Asayama K, Shirahata A . N-acetylcysteine attenuates TNF-alpha induced changes in secretion of interleukin-6, plasminogen activator inhibitor-1 and adiponectin from 3T3-L1 adipocytes. Life Sci. 2006; 79(25):2405-12. DOI: 10.1016/j.lfs.2006.08.004. View

Calzadilla P, Sapochnik D, Cosentino S, Diz V, Dicelio L, Calvo J . N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes. Int J Mol Sci. 2011; 12(10):6936-51. PMC: 3211019. DOI: 10.3390/ijms12106936. View

Castro J, Grune T, Speckmann B . The two faces of reactive oxygen species (ROS) in adipocyte function and dysfunction. Biol Chem. 2016; 397(8):709-24. DOI: 10.1515/hsz-2015-0305. View

Chang Y, Chuang L . The role of oxidative stress in the pathogenesis of type 2 diabetes: from molecular mechanism to clinical implication. Am J Transl Res. 2010; 2(3):316-31. PMC: 2892404. View

Choi J, Lee C, Lee J, Choi D, Sohng J, Park Y . 7,8-Dihydroxyflavone inhibits adipocyte differentiation via antioxidant activity and induces apoptosis in 3T3-L1 preadipocyte cells. Life Sci. 2015; 144:103-12. DOI: 10.1016/j.lfs.2015.11.028. View

Duthie G, Crozier A . Plant-derived phenolic antioxidants. Curr Opin Clin Nutr Metab Care. 2000; 3(6):447-51. DOI: 10.1097/00075197-200011000-00006. View

10.

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

11.

Evans J, Goldfine I, Maddux B, Grodsky G . Are oxidative stress-activated signaling pathways mediators of insulin resistance and beta-cell dysfunction?. Diabetes. 2002; 52(1):1-8. DOI: 10.2337/diabetes.52.1.1. View

12.

Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y . Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest. 2004; 114(12):1752-61. PMC: 535065. DOI: 10.1172/JCI21625. View

13.

Gomes A, Fernandes E, Lima J . Fluorescence probes used for detection of reactive oxygen species. J Biochem Biophys Methods. 2005; 65(2-3):45-80. DOI: 10.1016/j.jbbm.2005.10.003. View

14.

Han C, Umemoto T, Omer M, den Hartigh L, Chiba T, Leboeuf R . NADPH oxidase-derived reactive oxygen species increases expression of monocyte chemotactic factor genes in cultured adipocytes. J Biol Chem. 2012; 287(13):10379-10393. PMC: 3322984. DOI: 10.1074/jbc.M111.304998. View

15.

Hirota A, Taki S, Kawaii S, Yano M, Abe N . 1,1-Diphenyl-2-picrylhydrazyl radical-scavenging compounds from soybean miso and antiproliferative activity of isoflavones from soybean miso toward the cancer cell lines. Biosci Biotechnol Biochem. 2000; 64(5):1038-40. DOI: 10.1271/bbb.64.1038. View

16.

Jang M, Choi H, Kim G . Inhibitory effects of var. iwarenge extracts on reactive oxygen species production and lipid accumulation during 3T3-L1 adipocyte differentiation. Food Sci Biotechnol. 2019; 28(1):227-236. PMC: 6365340. DOI: 10.1007/s10068-018-0426-x. View

17.

Kakkar P, Das B, Viswanathan P . A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 1984; 21(2):130-2. View

18.

Kampa M, Alexaki V, Notas G, Nifli A, Nistikaki A, Hatzoglou A . Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: potential mechanisms of action. Breast Cancer Res. 2004; 6(2):R63-74. PMC: 400651. DOI: 10.1186/bcr752. View

19.

Koroleva O, Torkova A, Nikolaev I, Khrameeva E, Fedorova T, Tsentalovich M . Evaluation of the antiradical properties of phenolic acids. Int J Mol Sci. 2014; 15(9):16351-80. PMC: 4200783. DOI: 10.3390/ijms150916351. View

20.

Lee H, Lee Y, Choi H, Ko E, Kim J . Reactive oxygen species facilitate adipocyte differentiation by accelerating mitotic clonal expansion. J Biol Chem. 2009; 284(16):10601-9. PMC: 2667747. DOI: 10.1074/jbc.M808742200. View