Circular RNA PPP1CC Promotes -lipopolysaccharide-induced Pyroptosis of Vascular Smooth Muscle Cells by Activating the HMGB1/TLR9/AIM2 Pathway

Overview

Journal J Int Med Res

Publisher Sage Publications

Specialty General Medicine

Date 2021 Mar 26

PMID 33769113

Citations 14

Authors

Jie Liu

Yong Wang

Yaoyun Liao

Ying Zhou

Jijin Zhu

Affiliations

Soon will be listed here.

Abstract

Objective: () plays a critical role in the occurrence and development of atherosclerosis. Lipopolysaccharide from (-LPS) could lead to pyroptosis of vascular smooth muscle cells (VSMCs) and induce instability of atherosclerotic plaque. Therefore, pyroptosis of VSMCs could promote the process of atherosclerosis. However, the exact mechanism of -LPS-induced pyroptosis of VSMCs is unclear.

Methods: We determined pyroptosis and expression of interleukin (IL)-1β and IL-18 in VSMCs using 4',6-diamidino-2-phenylindole staining and ELISA after stimulation by -LPS. We established a knockdown plasmid containing the circular (circ)RNA PPP1CC and transfected it into VSMCs. Luciferase assays were performed to reveal the association between microRNAs miR-103a-3p and miR-107 and circRNA PPP1CC.

Results: Stimulation of -LPS led to pyroptosis of VSMCs. Knockdown of circRNA PPP1CC relieved the -LPS-induced pyroptosis of VSMCs and suppressed the expression of , , , and cleaved caspase-1. Luciferase assays showed that PPP1CC directly targeted and competitively adsorbed miR-103a-3p and miR-107, weakening the inhibitory effect of these microRNAs on the expression of .

Conclusion: Knockdown of circRNA PPP1CC relieved -LPS-induced pyroptosis of VSMCs. Pyroptosis of VSMCs appears to promote atherosclerosis and may represent a novel therapeutic target for its treatment.

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