Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Mar 25

PMID 33763049

Citations 26

Authors

Yongqi Mu

Hong Zeng

Wei Chen

Affiliations

Soon will be listed here.

Abstract

is an opportunistic pathogen, and its biofilm formation ability is an important virulent factor. Quercetin, a typical flavonoid ubiquitously used in dietary supplementation, is known for its antioxidant property, but its anti-biofilm activity against remains unknown. In this study, the anti-biofilm activity of quercetin was investigated using ATCC35984, a strong biofilm-positive strain. An attempt was made to disclose the mechanisms of the anti-biofilm activity of quercetin. exhibited a less cell surface hydrophobicity after quercetin treatment. Also, quercetin effectively inhibited cells from adhering to the glass slides. Quercetin downregulated the intercellular adhesion () locus and then polysaccharide intercellular adhesin (PIA) production was reduced. Therefore, cells became less hydrophobic, which supported quercetin's anti-biofilm effect. Our study suggests that quercetin from plants be given further attention as a potential anti-biofilm agent against the biofilm formation of , even biofilm infections of other bacteria.

Citing Articles

From Traditional Medicine to the Laboratory: A Multidisciplinary Investigation on L. Collected in Valle Imagna (BG, North of Italy).

Milani F, Muratore C, Biella S, Bottoni M, Rossi E, Colombo L Plants (Basel). 2025; 14(3).

PMID: 39942904 PMC: 11819889. DOI: 10.3390/plants14030340.

Biovalorization of whey waste as economic nutriment for mycogenic production of single cell oils with promising antibiofilm and anticancer potentiality.

Eltarahony M, El-Deeb N, Abu-Serie M, El-Shall H J Biol Eng. 2024; 18(1):62.

PMID: 39497156 PMC: 11533293. DOI: 10.1186/s13036-024-00455-y.

lysozyme inhibitor LprI mediated by HmsP and c-di-GMP is essential for biofilm formation and virulence.

Ji X, Fan D, Wang J, Zhang B, Hu Y, Lv H Appl Environ Microbiol. 2024; 90(10):e0156424.

PMID: 39297664 PMC: 11497839. DOI: 10.1128/aem.01564-24.

Organ-Specific Metabolomics of Aerial Parts of Melochia corchorifolia for Antibiofilm and Wound Healing Activities.

Mahendran S, Mohanapriya V, Wilson J, Swain D, Ramalingam V Appl Biochem Biotechnol. 2024; 197(2):691-713.

PMID: 39215903 DOI: 10.1007/s12010-024-05053-8.

The Role of Quercetin as a Plant-Derived Bioactive Agent in Preventive Medicine and Treatment in Skin Disorders.

Zaborowski M, Dlugosz A, Blaszak B, Szulc J, Leis K Molecules. 2024; 29(13).

PMID: 38999158 PMC: 11243040. DOI: 10.3390/molecules29133206.

References

Mello P, Riboli D, de Almeida Martins L, Brito M, Victoria C, Romero L . spp. Isolated from Bovine Subclinical Mastitis in Different Regions of Brazil: Molecular Typing and Biofilm Gene Expression Analysis by RT-qPCR. Antibiotics (Basel). 2020; 9(12). PMC: 7764751. DOI: 10.3390/antibiotics9120888. View

Annapoorani A, Parameswari R, Pandian S, Veera Ravi A . Methods to determine antipathogenic potential of phenolic and flavonoid compounds against urinary pathogen Serratia marcescens. J Microbiol Methods. 2012; 91(1):208-11. DOI: 10.1016/j.mimet.2012.06.007. View

Rohde H, Burdelski C, Bartscht K, Hussain M, Buck F, Horstkotte M . Induction of Staphylococcus epidermidis biofilm formation via proteolytic processing of the accumulation-associated protein by staphylococcal and host proteases. Mol Microbiol. 2005; 55(6):1883-95. DOI: 10.1111/j.1365-2958.2005.04515.x. View

Boots A, Haenen G, Bast A . Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol. 2008; 585(2-3):325-37. DOI: 10.1016/j.ejphar.2008.03.008. View

Vadyvaloo V, Otto M . Molecular genetics of Staphylococcus epidermidis biofilms on indwelling medical devices. Int J Artif Organs. 2005; 28(11):1069-78. DOI: 10.1177/039139880502801104. View

Thompson K, Freitag L, Styger U, Camenisch K, Zeiter S, Arens D . Impact of low bone mass and antiresorptive therapy on antibiotic efficacy in a rat model of orthopedic device-related infection. J Orthop Res. 2020; 39(2):415-425. DOI: 10.1002/jor.24951. View

Gomes F, Saavedra M, Henriques M . Bovine mastitis disease/pathogenicity: evidence of the potential role of microbial biofilms. Pathog Dis. 2016; 74(3). DOI: 10.1093/femspd/ftw006. View

Nithyanand P, Thenmozhi R, Rathna J, Pandian S . Inhibition of Streptococcus pyogenes biofilm formation by coral-associated actinomycetes. Curr Microbiol. 2009; 60(6):454-60. DOI: 10.1007/s00284-009-9564-y. View

Pereira M, Grubbs C, Barnes L, Li H, Olson G, Eto I . Effects of the phytochemicals, curcumin and quercetin, upon azoxymethane-induced colon cancer and 7,12-dimethylbenz[a]anthracene-induced mammary cancer in rats. Carcinogenesis. 1996; 17(6):1305-11. DOI: 10.1093/carcin/17.6.1305. View

10.

Lee J, Kim Y, Lee K, Kim C, Park D, Ju Y . Streptomyces-derived actinomycin D inhibits biofilm formation by Staphylococcus aureus and its hemolytic activity. Biofouling. 2016; 32(1):45-56. DOI: 10.1080/08927014.2015.1125888. View

11.

Pratt L, Kolter R . Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol. 1998; 30(2):285-93. DOI: 10.1046/j.1365-2958.1998.01061.x. View

12.

Halasa T, Huijps K, Osteras O, Hogeveen H . Economic effects of bovine mastitis and mastitis management: a review. Vet Q. 2007; 29(1):18-31. DOI: 10.1080/01652176.2007.9695224. View

13.

Fernandes J, Zanardo L, Galvao N, Carvalho I, Nero L, Moreira M . Escherichia coli from clinical mastitis: serotypes and virulence factors. J Vet Diagn Invest. 2012; 23(6):1146-52. DOI: 10.1177/1040638711425581. View

14.

Mack D, Siemssen N, Laufs R . Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion. Infect Immun. 1992; 60(5):2048-57. PMC: 257114. DOI: 10.1128/iai.60.5.2048-2057.1992. View

15.

Rojas-Lema S, Torres-Giner S, Quiles-Carrillo L, Gomez-Caturla J, Garcia-Garcia D, Balart R . On the Use of Phenolic Compounds Present in Citrus Fruits and Grapes as Natural Antioxidants for Thermo-Compressed Bio-Based High-Density Polyethylene Films. Antioxidants (Basel). 2020; 10(1). PMC: 7823819. DOI: 10.3390/antiox10010014. View

16.

Kannappan A, Durgadevi R, Srinivasan R, Lagoa R, Packiavathy I, Pandian S . 2-Hydroxy-4-methoxybenzaldehyde from is antagonistic to biofilm formation. Biofouling. 2020; 36(5):549-563. DOI: 10.1080/08927014.2020.1777989. View

17.

Okada A, Tabata H, Shoda M, Shoin W, Kobayashi H, Okano T . Safe and effective transvenous lead extraction for elderly patients utilizing non-laser and laser tools: a single-center experience in Japan. Heart Vessels. 2021; 36(6):882-889. DOI: 10.1007/s00380-020-01761-3. View

18.

Liduma I, Tracevska T, Bers U, Zilevica A . Phenotypic and genetic analysis of biofilm formation by Staphylococcus epidermidis. Medicina (Kaunas). 2012; 48(6):305-9. View

19.

Jin X, Zhao S . Extraction optimization and bioactivities of an extracellular polysaccharide produced by Aspergillus fumigatus. Int J Biol Macromol. 2014; 68:13-7. DOI: 10.1016/j.ijbiomac.2014.04.024. View

20.

Mu Y, Xie T, Zeng H, Chen W, Wan C, Zhang L . Streptomyces-derived actinomycin D inhibits biofilm formation via downregulating ica locus and decreasing production of PIA in Staphylococcus epidermidis. J Appl Microbiol. 2019; 128(4):1201-1207. DOI: 10.1111/jam.14543. View