Identification of Novel Juvenile-hormone Signaling Activators High-throughput Screening with a Chemical Library

Overview

Journal J Pestic Sci

Date 2021 Mar 22

PMID 33746546

Citations 1

Authors

Takumi Kayukawa

Kenjiro Furuta

Kiyoaki Yonesu

Takayoshi Okabe

Affiliations

Soon will be listed here.

Abstract

Juvenile hormone (JH) is an insect-specific hormone that regulates molting and metamorphosis. Hence, JH signaling inhibitors (JHSIs) and activators (JHSAs) can be used as effective insect growth regulators (IGRs) for pest management. In our previous study, we established a high-throughput screening (HTS) system for exploration of novel JHSIs and JHSAs using a cell line (BmN_JF&AR cells) and succeeded in identifying novel JHSIs from a chemical library. Here, we searched for novel JHSAs using this system. The four-step HTS yielded 10 compounds as candidate JHSAs; some of these compounds showed novel basic structures, whereas the others were composed of a 4-phenoxyphenoxymethyl skeleton, the basic structure of several existing JH analogs (pyriproxyfen and fenoxycarb). Topical application of seven compounds to larvae significantly prolonged the larval period, suggesting that the identified JHSAs may be promising IGRs targeting the JH signaling pathway.

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