Inactivation Effects and Mechanisms of Plasma-activated Water Combined with Sodium Laureth Sulfate (SLES) Against Saccharomyces Cerevisiae

Overview

Journal Appl Microbiol Biotechnol

Specialties Biomedical Engineering
Biotechnology
Microbiology

Date 2021 Mar 19

PMID 33738554

Citations 3

Authors

Xiao Liu

Yunfei Li

Rong Zhang

Lulu Huangfu

Guihong Du

Qisen Xiang

Affiliations

Soon will be listed here.

Abstract

The present study aimed to elucidate the antifungal effect and underlying mechanism of plasma-activated water (PAW) combined with sodium laureth sulfate (SLES) against Saccharomyces cerevisiae. S. cerevisiae, initially at 6.95 log colony-forming unit (CFU)/mL, decreased to an undetectable level following the synergistic treatment of PAW and SLES (0.50 mg/mL) for 20 min. After PAW treatment combined with SLES (2.5 mg/mL) for 30 min, the S. cerevisiae cells on polyethylene films also reduced to an undetectable level from the initial load of 5.84 log CFU/cm. PAW + SLES treatment caused severe disruption of membrane integrity and increased lipid oxidation within the cell membrane and the intracellular reactive oxygen species levels in S. cerevisiae cells. Besides, the disruption of the mitochondrial membrane potential (∆ψm) was also observed in S. cerevisiae cells after treatment of PAW and SLES at 0.01 mg/mL for 5 min. These data suggest that the combined treatment of PAW and SLES causes oxidation injury to cell membranes and abnormal ∆ψm in S. cerevisiae, which may be eventually responsible for cell death. This study demonstrates the potential application of PAW combined with SLES as an alternative disinfection method. Key Points • PAW + SLES exhibited synergistic antifungal activity against S. cerevisiae. • PAW + SLES resulted in severe disruption of membrane integrity and permeability. • PAW + SLES induced accumulation of reactive oxygen species in S. cerevisiae cells.

Citing Articles

Enhancement effect of carvacrol on yeast inactivation by mild pressure carbon dioxide.

Niu L, Wu Z, Liu J, Xiang Q, Bai Y Arch Microbiol. 2023; 205(11):353.

PMID: 37815591 DOI: 10.1007/s00203-023-03689-4.

Plasma activated Ezhangfeng Cuji as innovative antifungal agent and its inactivation mechanism.

Lin L, Zhuo Y, Dong Q, Yang C, Cheng C, Liu T AMB Express. 2023; 13(1):65.

PMID: 37368076 PMC: 10299983. DOI: 10.1186/s13568-023-01571-6.

Effect of Combined Treatment with Cinnamon Oil and -High Pressure CO against .

Niu L, Liu J, Wang X, Wu Z, Xiang Q, Bai Y Foods. 2022; 11(21).

PMID: 36360087 PMC: 9658994. DOI: 10.3390/foods11213474.

References

Atale N, Gupta S, Yadav U, Rani V . Cell-death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques. J Microsc. 2014; 255(1):7-19. DOI: 10.1111/jmi.12133. View

Bai Y, Muhammad A, Hu Y, Koseki S, Liao X, Chen S . Inactivation kinetics of Bacillus cereus spores by Plasma activated water (PAW). Food Res Int. 2020; 131:109041. DOI: 10.1016/j.foodres.2020.109041. View

Bintsis T . Foodborne pathogens. AIMS Microbiol. 2019; 3(3):529-563. PMC: 6604998. DOI: 10.3934/microbiol.2017.3.529. View

Frias E, Iglesias Y, Alvarez-Ordonez A, Prieto M, Gonzalez-Raurich M, Lopez M . Evaluation of Cold Atmospheric Pressure Plasma (CAPP) and plasma-activated water (PAW) as alternative non-thermal decontamination technologies for tofu: Impact on microbiological, sensorial and functional quality attributes. Food Res Int. 2020; 129:108859. DOI: 10.1016/j.foodres.2019.108859. View

Guo L, Xu R, Gou L, Liu Z, Zhao Y, Liu D . Mechanism of Virus Inactivation by Cold Atmospheric-Pressure Plasma and Plasma-Activated Water. Appl Environ Microbiol. 2018; 84(17). PMC: 6102979. DOI: 10.1128/AEM.00726-18. View

Heredia N, Garcia S . Animals as sources of food-borne pathogens: A review. Anim Nutr. 2018; 4(3):250-255. PMC: 6116329. DOI: 10.1016/j.aninu.2018.04.006. View

Kalyanaraman B, Darley-Usmar V, Davies K, Dennery P, Forman H, Grisham M . Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free Radic Biol Med. 2011; 52(1):1-6. PMC: 3911769. DOI: 10.1016/j.freeradbiomed.2011.09.030. View

Kamgang-Youbi G, Herry J, Meylheuc T, Brisset J, Bellon-Fontaine M, Doubla A . Microbial inactivation using plasma-activated water obtained by gliding electric discharges. Lett Appl Microbiol. 2009; 48(1):13-8. DOI: 10.1111/j.1472-765X.2008.02476.x. View

Kramer B, Hasse D, Guist S, Schmitt-John T, Muranyi P . Inactivation of bacterial endospores on surfaces by plasma processed air. J Appl Microbiol. 2019; 128(4):920-933. DOI: 10.1111/jam.14528. View

10.

Lacombe A, Niemira B, Gurtler J, Kingsley D, Li X, Chen H . Surfactant-Enhanced Organic Acid Inactivation of Tulane Virus, a Human Norovirus Surrogate. J Food Prot. 2018; 81(2):279-283. DOI: 10.4315/0362-028X.JFP-17-330. View

11.

Liao X, Muhammad A, Chen S, Hu Y, Ye X, Liu D . Bacterial spore inactivation induced by cold plasma. Crit Rev Food Sci Nutr. 2018; 59(16):2562-2572. DOI: 10.1080/10408398.2018.1460797. View

12.

Los A, Ziuzina D, Boehm D, Cullen P, Bourke P . Inactivation Efficacies and Mechanisms of Gas Plasma and Plasma-Activated Water against Aspergillus flavus Spores and Biofilms: a Comparative Study. Appl Environ Microbiol. 2020; 86(9). PMC: 7170485. DOI: 10.1128/AEM.02619-19. View

13.

Okimotoa Y, Watanabea A, Nikia E, Yamashitab T, Noguchia N . A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett. 2000; 474(2-3):137-40. DOI: 10.1016/s0014-5793(00)01587-8. View

14.

Royintarat T, Choi E, Boonyawan D, Seesuriyachan P, Wattanutchariya W . Chemical-free and synergistic interaction of ultrasound combined with plasma-activated water (PAW) to enhance microbial inactivation in chicken meat and skin. Sci Rep. 2020; 10(1):1559. PMC: 6994601. DOI: 10.1038/s41598-020-58199-w. View

15.

Rozali S, Milani E, Deed R, Silva F . Bacteria, mould and yeast spore inactivation studies by scanning electron microscope observations. Int J Food Microbiol. 2017; 263:17-25. DOI: 10.1016/j.ijfoodmicro.2017.10.008. View

16.

Sivandzade F, Bhalerao A, Cucullo L . Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe. Bio Protoc. 2019; 9(1). PMC: 6343665. DOI: 10.21769/BioProtoc.3128. View

17.

Takahashi M, Shibata M, Niki E . Estimation of lipid peroxidation of live cells using a fluorescent probe, diphenyl-1-pyrenylphosphine. Free Radic Biol Med. 2001; 31(2):164-74. DOI: 10.1016/s0891-5849(01)00575-5. View

18.

Wang T, Libardo M, Angeles-Boza A, Pellois J . Membrane Oxidation in Cell Delivery and Cell Killing Applications. ACS Chem Biol. 2017; 12(5):1170-1182. PMC: 5905413. DOI: 10.1021/acschembio.7b00237. View

19.

Xiang Q, Liu X, Li J, Liu S, Zhang H, Bai Y . Effects of dielectric barrier discharge plasma on the inactivation of Zygosaccharomyces rouxii and quality of apple juice. Food Chem. 2018; 254:201-207. DOI: 10.1016/j.foodchem.2018.02.008. View

20.

Yost A, Joshi S . Atmospheric Nonthermal Plasma-Treated PBS Inactivates Escherichia coli by Oxidative DNA Damage. PLoS One. 2015; 10(10):e0139903. PMC: 4603800. DOI: 10.1371/journal.pone.0139903. View