Evaluating the Potential of an Amelogenin-derived Peptide in Tertiary Dentin Formation

Overview

Journal Regen Biomater

Specialty Biomedical Engineering

Date 2021 Mar 19

PMID 33738118

Citations 2

Authors

Xiu Peng

Sili Han

Kun Wang

Longjiang Ding

Zhenqi Liu

Linglin Zhang

Affiliations

Soon will be listed here.

Abstract

Several novel biomaterials have been developed for dental pulp capping by inducing tertiary dentin formation. The aim of this study was to evaluate the effect of QP5, an amelogenin-based peptide, on the mineralization of dental pulp cells (DPCs) and . The cell viability of human DPCs (hDPCs) after treatment with QP5 was determined using the Cell Counting Kit-8 (CCK-8). Migration of hDPCs was assessed using scratch assays, and the pro-mineralization effect was determined using alkaline phosphatase (ALP) staining, alizarin red staining and the expression of mineralization-related genes and proteins. The results showed that QP5 had little effect on the cell viability, and significantly enhanced the migration capability of hDPCs. QP5 promoted the formation of mineralized nodules, and upregulated the activity of ALP, the expression of mRNA and proteins of mineralization-related genes. A pulp capping model in rats was generated to investigate the biological effect of QP5. The results of micro-computed tomography and haematoxylin and eosin staining indicated that the formation of tertiary dentin in QP5-capping groups was more prominent than that in the negative control group. These results indicated the potential of QP5 as a pulp therapy agent.

Citing Articles

Physiologic dentin regeneration: its past, present, and future perspectives.

Lee M, Lee Y, Shon W, Park J Front Physiol. 2023; 14:1313927.

PMID: 38148896 PMC: 10750396. DOI: 10.3389/fphys.2023.1313927.

An amelogenin-based peptide hydrogel promoted the odontogenic differentiation of human dental pulp cells.

Li X, Yu Z, Jiang S, Dai X, Wang G, Wang Y Regen Biomater. 2022; 9:rbac039.

PMID: 35936553 PMC: 9348551. DOI: 10.1093/rb/rbac039.

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