Silencing of Circular RNA_0000326 Inhibits Cervical Cancer Cell Proliferation, Migration and Invasion by Boosting MicroRNA-338-3p-dependent Down-regulation of CDK4

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2021 Mar 18

PMID 33735107

Citations 9

Authors

Zhaoxin Wang

Chenchen Ren

Li Yang

Xiaoan Zhang

Jiaxi Liu

Yuanhang Zhu

Dongyuan Jiang

Affiliations

Soon will be listed here.

Abstract

Cervical cancer is one of the leading causes of cancer-related death among women, which is attributed partly by limited treatment options. Recent studies have provided in-depth explanations regarding the role of circular RNA in cancers. We aimed to investigate the role of circular RNA_0000326 in cervical cancer. Bioinformatics analysis revealed a high circ_0000326 expression in cervical cancer. Cervical cancer cells and tissues were also observed to have elevated levels of circ_0000326 and the upregulation of circ_0000326 depended on the stage of cancer. Transfection with siRNA of circ_0000326 resulted in the inhibition of proliferation, migration and cell cycle of cancer cells. Interestingly, we confirmed that circ_0000326 served as a sponge for microRNA-338-3p and that the miRNA bound to Cyclin-dependent kinase 4. In the presence of microRNA-338-3p mimic or silencing of circ_0000326, Cyclin-dependent kinase 4 expression was decreased. Transfection with microRNA-338-3p mimic inhibited cell clone formation and proliferation. Moreover, experiment revealed that the injection of shRNA-circ_0000326 lentivirus suppressed tumor growth and decreased Cyclin-dependent kinase 4 expression. Taken altogether, our results showed that circ_0000326 exerted oncogenic effects on cervical cancer by upregulating Cyclin-dependent kinase 4 sponging microRNA-338-3p. This systematic investigation on circ_0000326 could provide further insight into cervical cancer.

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