Genetic Assessment of the Effects of Self-fertilization in a L. Hybrids Using Molecular Cytogenetic Methods (FISH and ISSR)

Overview

Journal Saudi J Biol Sci

Specialty Biology

Date 2021 Mar 18

PMID 33732061

Authors

Fahad Ramzan

Hyoung Tae Kim

Adnan Younis

Yasir Ramzan

Ki-Byung Lim

Affiliations

Soon will be listed here.

Abstract

Self-fertilization (also termed selfing) is a mode of reproduction that occurs in hermaphrodites and has evolved several times in various plant and animal species. A transition from outbreeding to selfing in hermaphroditic flowers is typically associated with changes in flower morphology and functionality. This study aimed to identify genetic effects of selfing in the F2 progeny of F1 hybrid developed by crossing with the Asiatic hybrid 'Dreamland.' Fluorescence hybridization (FISH) and inter-simple sequence repeats (ISSR) techniques were used to detect genetic variations in plants produced by selfing. The FISH results showed that F1 hybrid were similar to the female parent () regarding the 45S loci, but F2 individuals showed variation in the number and location of the respective loci. In F2 progeny, F2-2, F2-3, F2-4, F2-5, and F2-8 hybrids expressed two strong and one weak 5S signal on chromosome 3, whereas F2-7 and F2-9 individuals expressed one strong and two weak signals. Only two strong 5S signals were detected in an F2-1 plant. The ISSR results showed a maximum similarity value of 0.6269 between the female parent and the F2-2 hybrid. Regarding similarity to the male parent, a maximum value of 0.6119 was found in the F2-1 and F2-2 hybrids. The highest genetic distance from and the Asiatic hybrid 'Dreamland' was observed in the F2-4 progeny (0.6352 and 0.7547, respectively). Phylogenetic relationships showed that the F2 progeny were closer to the male parent than to the female parent. Self-fertilization showed effects on variation among the F2 progeny, and effects on the genome were confirmed using FISH and ISSR analyses.

References

Biswas M, Nath U, Howlader J, Bagchi M, Natarajan S, Kayum M . Exploration and Exploitation of Novel SSR Markers for Candidate Transcription Factor Genes in Lilium Species. Genes (Basel). 2018; 9(2). PMC: 5852593. DOI: 10.3390/genes9020097. View

Steele K, Edwards G, Zhu J, Witcombe J . Marker-evaluated selection in rice: shifts in allele frequency among bulks selected in contrasting agricultural environments identify genomic regions of importance to rice adaptation and breeding. Theor Appl Genet. 2004; 109(6):1247-60. DOI: 10.1007/s00122-004-1732-7. View

Zhang D, Yang Q, Bao W, Zhang Y, Han B, Xue Y . Molecular cytogenetic characterization of the Antirrhinum majus genome. Genetics. 2004; 169(1):325-35. PMC: 1448859. DOI: 10.1534/genetics.104.031146. View

Ingvarsson P . A metapopulation perspective on genetic diversity and differentiation in partially self-fertilizing plants. Evolution. 2003; 56(12):2368-73. DOI: 10.1111/j.0014-3820.2002.tb00162.x. View

Barrett S . Understanding plant reproductive diversity. Philos Trans R Soc Lond B Biol Sci. 2009; 365(1537):99-109. PMC: 2842705. DOI: 10.1098/rstb.2009.0199. View

Younis A, Ramzan F, Hwang Y, Lim K . FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants. Plant Cell Rep. 2015; 34(9):1477-88. DOI: 10.1007/s00299-015-1828-3. View

Charlesworth D, Wright S . Breeding systems and genome evolution. Curr Opin Genet Dev. 2001; 11(6):685-90. DOI: 10.1016/s0959-437x(00)00254-9. View

Kumar R, Parthiban K, Govinda Rao M . Molecular characterization of Jatropha genetic resources through inter-simple sequence repeat (ISSR) markers. Mol Biol Rep. 2008; 36(7):1951-6. DOI: 10.1007/s11033-008-9404-3. View

Jiang J, Gill B . Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research. Genome. 2006; 49(9):1057-68. DOI: 10.1139/g06-076. View

10.

Nybom H . Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol. 2004; 13(5):1143-55. DOI: 10.1111/j.1365-294X.2004.02141.x. View

11.

Hernandez-Verdun D . Structural organization of the nucleolus in mammalian cells. Methods Achiev Exp Pathol. 1986; 12:26-62. View

12.

Huang J, Ma L, Yang F, Fei S, Li L . 45S rDNA regions are chromosome fragile sites expressed as gaps in vitro on metaphase chromosomes of root-tip meristematic cells in Lolium spp. PLoS One. 2008; 3(5):e2167. PMC: 2366065. DOI: 10.1371/journal.pone.0002167. View

13.

Nei M, Li W . Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979; 76(10):5269-73. PMC: 413122. DOI: 10.1073/pnas.76.10.5269. View

14.

Guillen A, Hirai Y, Tanoue T, Hirai H . Transcriptional repression mechanisms of nucleolus organizer regions (NORs) in humans and chimpanzees. Chromosome Res. 2004; 12(3):225-37. DOI: 10.1023/b:chro.0000021911.43225.eb. View

15.

Soltis D, Soltis P . The role of phylogenetics in comparative genetics. Plant Physiol. 2003; 132(4):1790-800. PMC: 526274. DOI: 10.1104/pp.103.022509. View

16.

Wang J, Yang Y, Liu X, Huang J, Wang Q, Gu J . Transcriptome profiling of the cold response and signaling pathways in Lilium lancifolium. BMC Genomics. 2014; 15:203. PMC: 4003810. DOI: 10.1186/1471-2164-15-203. View

17.

Nei M . Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci U S A. 1973; 70(12):3321-3. PMC: 427228. DOI: 10.1073/pnas.70.12.3321. View

18.

Khajudparn P, Prajongjai T, Poolsawat O, Tantasawat P . Application of ISSR markers for verification of F₁ hybrids in mungbean (Vigna radiata). Genet Mol Res. 2012; 11(3):3329-38. DOI: 10.4238/2012.September.17.3. View

19.

Barrett S . The evolution of plant sexual diversity. Nat Rev Genet. 2002; 3(4):274-84. DOI: 10.1038/nrg776. View

20.

Schubert I . Chromosome evolution. Curr Opin Plant Biol. 2007; 10(2):109-15. DOI: 10.1016/j.pbi.2007.01.001. View