Global Mapping of Glycosylation Pathways in Human-derived Cells

Overview

Journal Dev Cell

Publisher Cell Press

Specialties Cell Biology
Reproductive Medicine

Date 2021 Mar 17

PMID 33730547

Citations 42

Authors

Yi-Fan Huang

Kazuhiro Aoki

Sachiko Akase

Mayumi Ishihara

Yi-Shi Liu

Ganglong Yang

Yasuhiko Kizuka

Shuji Mizumoto

Michael Tiemeyer

Xiao-Dong Gao

Kiyoko F Aoki-Kinoshita

Morihisa Fujita

Affiliations

Soon will be listed here.

Abstract

Glycans are one of the fundamental classes of macromolecules and are involved in a broad range of biological phenomena. A large variety of glycan structures can be synthesized depending on tissue or cell types and environmental changes. Here, we developed a comprehensive glycosylation mapping tool, termed GlycoMaple, to visualize and estimate glycan structures based on gene expression. We informatically selected 950 genes involved in glycosylation and its regulation. Expression profiles of these genes were mapped onto global glycan metabolic pathways to predict glycan structures, which were confirmed using glycomic analyses. Based on the predictions of N-glycan processing, we constructed 40 knockout HEK293 cell lines and analyzed the effects of gene knockout on glycan structures. Finally, the glycan structures of 64 cell lines, 37 tissues, and primary colon tumor tissues were estimated and compared using publicly available databases. Our systematic approach can accelerate glycan analyses and engineering in mammalian cells.

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