Anaerocolumna Sedimenticola Sp. Nov., Isolated from Fresh Water Sediment

Strain CBA3638 was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638 were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5-1.0 μm wide, and 4.0-4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638 belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6-95.0%). The DDH value with A. cellulosilytica SN021 showed 15.0% relatedness. The genome of strain CBA3638 consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G + C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C and C and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638 as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638 (= KACC 21652 = DSM 110663).