Cell Wall N-glycan of Candida Albicans Ameliorates Early Hyper- and Late Hypo-immunoreactivity in Sepsis

Overview

Journal Commun Biol

Specialty Biology

Date 2021 Mar 17

PMID 33727664

Citations 6

Authors

Masataka Kawakita

Taiki Oyama

Ikuma Shirai

Shuto Tanaka

Kotaro Akaki

Shinya Abe

Takuma Asahi

Guangwei Cui

Fumie Itoh

Masato Sasaki

Nobuyuki Shibata

Koichi Ikuta

Tomomitsu Hatakeyama

Kazuhiko Takahara

Affiliations

Soon will be listed here.

Abstract

Severe infection often causes a septic cytokine storm followed by immune exhaustion/paralysis. Not surprisingly, many pathogens are equipped with various anti-inflammatory mechanisms. Such mechanisms might be leveraged clinically to control septic cytokine storms. Here we show that N-glycan from pathogenic C. albicans ameliorates mouse sepsis through immunosuppressive cytokine IL-10. In a sepsis model using lipopolysaccharide (LPS), injection of the N-glycan upregulated serum IL-10, and suppressed pro-inflammatory IL-1β, TNF-α and IFN-γ. The N-glycan also improved the survival of mice challenged by LPS. Analyses of structurally defined N-glycans from several yeast strains revealed that the mannose core is key to the upregulation of IL-10. Knocking out the C-type lectin Dectin-2 abrogated the N-glycan-mediated IL-10 augmentation. Furthermore, C. albicans N-glycan ameliorated immune exhaustion/immune paralysis after acute inflammation. Our results suggest a strategy where the immunosuppressive mechanism of one pathogen can be applied to attenuate a severe inflammation/cytokine storm caused by another pathogen.

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