Respiration-dependent Contraction of Swollen Heart Mitochondria: Participation of the K+/H+ Antiporter

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 1988 Apr 1

PMID 3372495

Citations 4

Authors

G P Brierley

M H Davis

D W Jung

Affiliations

Soon will be listed here.

Abstract

Respiration-dependent contraction of heart mitochondria swollen passively in K+ nitrate is activated by the ionophore A23187 and inhibited by Mg2+. Ion extrusion and osmotic contraction under these conditions are strongly inhibited by quinine, a known inhibitor of the mitochondrial K+/H+ antiporter, as measured in other systems. The inhibition by quinine is relieved by the exogenous antiporter nigericin. Respiration-dependent contraction is also inhibited by dicyclohexylcarbodiimide (DCCD) when reacted under conditions known to inhibit K+/H+ antiport (Martin et al., J. Biol. Chem. 259, 2062-2065, 1984). These studies strongly support the concept that K+ is extruded from the matrix by the endogenous K+/H+ antiporter and that inhibition of this component by quinine or DCCD inhibits respiration-dependent contraction. The extrusion of K+ nitrate is accompanied by a respiration-dependent efflux of a considerable portion of the endogenous Mg2+. This Mg2+ efflux does not occur in the presence of nigericin or when the mitochondrial Na+/H+ antiporter is active. Mg2+ efflux may take place on the K+/H+ antiporter. DCCD, reacted under conditions that do not result in inhibition of the K+/H+ antiporter, blocks a monovalent cation uniport pathway. This uniport contributes to futile cation cycling at elevated pH, and its inhibition by DCCD stimulates respiration-dependent contraction.

Citing Articles

Tl(+) induces both cationic and transition pore permeability in the inner membrane of rat heart mitochondria.

Korotkov S, Nesterov V, Brailovskaya I, Furaev V, Novozhilov A J Bioenerg Biomembr. 2013; 45(6):531-9.

PMID: 24057477 DOI: 10.1007/s10863-013-9526-8.

Cation transport systems in mitochondria: Na+ and K+ uniports and exchangers.

Brierley G, Baysal K, Jung D J Bioenerg Biomembr. 1994; 26(5):519-26.

PMID: 7896767 DOI: 10.1007/BF00762736.

K+/H+ antiport in mitochondria.

Brierley G, Jung D J Bioenerg Biomembr. 1988; 20(2):193-209.

PMID: 3286632 DOI: 10.1007/BF00768394.

Measurement of matrix free Mg2+ concentration in rat heart mitochondria by using entrapped fluorescent probes.

Rutter G, Osbaldeston N, McCormack J, Denton R Biochem J. 1990; 271(3):627-34.

PMID: 2244870 PMC: 1149608. DOI: 10.1042/bj2710627.

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