Evaluation of Fecal Immunoassays for Canine Echinococcus Infection in China

Overview

Journal PLoS Negl Trop Dis

Specialties Infectious Diseases
Tropical Medicine

Date 2021 Mar 15

PMID 33720943

Citations 6

Authors

Liying Wang

Qian Wang

Huixia Cai

Hu Wang

Yan Huang

Yu Feng

Xuefei Bai

Min Qin

Sylvie Manguin

Laurent Gavotte

Weiping Wu

Roger Frutos

Affiliations

Soon will be listed here.

Abstract

Human echinococcosis is present worldwide but it is in China that disease prevalence is the highest. In western China, especially in the Tibetan Plateau, the burden of echinococcosis is the most important. Dogs are a major definitive host of Echinococcus and monitoring the presence of Echinococcus worms in dogs is therefore essential to efficiently control the disease. Detection kits based on three different technologies including sandwich ELISA, (indirect) ELISA, and gold immunodiffusion, are currently marketed and used in China. The objective of this work was to assess the efficacy of these kits, in particular with respect to sensitivity and specificity. Four fecal antigen detection kits for canine infection reflecting the three technologies were obtained from companies and tested in parallel on 220 fecal samples. The results indicate that the performance is lower than expected, in particular in terms of sensitivity. The best results were obtained with the sandwich ELISA technology. The gold immunofiltration yielded the poorest results. In all cases, further development is needed to improve the performance of these kits which are key components for the control of echinococcosis.

Citing Articles

Development of a Copro-RPA-CRISPR/Cas12a assay to detect Echinococcus granulosus nucleic acids isolated from canine feces using NaOH-based DNA extraction method.

Shao G, Zhu X, Hua R, Chen Y, Yang G PLoS Negl Trop Dis. 2024; 18(12):e0012753.

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Rapid Discriminative Identification of the Two Predominant Echinococcus Species from Canine Fecal Samples in the Tibetan Region of China by Loop-Mediated Isothermal Amplification-Lateral Flow Dipstick Assay.

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Assessment of echinococcosis control in Tibet Autonomous Region, China.

Wang L, Gongsang Q, Pang H, Qin M, Wang Y, Li J Infect Dis Poverty. 2022; 11(1):59.

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Geographic distribution and prevalence of human echinococcosis at the township level in the Tibet Autonomous Region.

Wang L, Quzhen G, Qin M, Liu Z, Pang H, Frutos R Infect Dis Poverty. 2022; 11(1):10.

PMID: 35063031 PMC: 8780799. DOI: 10.1186/s40249-022-00933-9.

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