Applications of Nanoparticle-Antibody Conjugates in Immunoassays and Tumor Imaging

Overview

Journal AAPS J

Specialty Pharmacology

Date 2021 Mar 15

PMID 33718979

Citations 10

Authors

Xinhao Lin

Andre OReilly Beringhs

Xiuling Lu

Affiliations

Soon will be listed here.

Abstract

Modern diagnostic technologies rely on both in vitro and in vivo modalities to provide a complete understanding of the clinical state of a patient. Nanoparticle-antibody conjugates have emerged as promising systems to confer increased sensitivity and accuracy for in vitro diagnostics (e.g., immunoassays). Meanwhile, in vivo applications have benefited from the targeting ability of nanoparticle-antibody conjugates, as well as payload flexibility and tailored biodistribution. This review provides an encompassing overview of nanoparticle-antibody conjugates, from chemistry to applications in medical immunoassays and tumor imaging, highlighting the underlying principles and unique features of relevant preclinical applications employing commonly used imaging modalities (e.g., optical/photoacoustics, positron-emission tomography, magnetic resonance imaging, X-ray computed tomography).

Citing Articles

Surface Functionalization of Nanocarriers with Anti-EGFR Ligands for Cancer Active Targeting.

Spada A, Gerber-Lemaire S Nanomaterials (Basel). 2025; 15(3).

PMID: 39940134 PMC: 11820047. DOI: 10.3390/nano15030158.

Emerging approaches for T cell-stimulating platform development.

Ariail E, Garcia Espinoza N, Stephenson A, Spangler J Cell Syst. 2024; 15(12):1198-1208.

PMID: 39701036 PMC: 11665896. DOI: 10.1016/j.cels.2024.11.007.

Tumor versus Tumor Cell Targeting in Metal-Based Nanoparticles for Cancer Theranostics.

Urbano-Gamez J, Guzzi C, Bernal M, Solivera J, Martinez-Zubiaurre I, Caro C Int J Mol Sci. 2024; 25(10).

PMID: 38791253 PMC: 11121233. DOI: 10.3390/ijms25105213.

GcForest-based compound-protein interaction prediction model and its application in discovering small-molecule drugs targeting CD47.

Shan W, Chen L, Xu H, Zhong Q, Xu Y, Yao H Front Chem. 2023; 11:1292869.

PMID: 37927570 PMC: 10623438. DOI: 10.3389/fchem.2023.1292869.

Pd@Pt nanoparticle-linked immunosorbent assay for quantification of Collagen type II.

Kwon E, Abusharkh H, Ruan X, Du D, Hammond-Pereira E, Van Wie B Anal Chim Acta. 2023; 1266:341265.

PMID: 37244654 PMC: 10564550. DOI: 10.1016/j.aca.2023.341265.

References

Cammarata C, Hughes M, Ofner 3rd C . Carbodiimide induced cross-linking, ligand addition, and degradation in gelatin. Mol Pharm. 2015; 12(3):783-93. PMC: 5585870. DOI: 10.1021/mp5006118. View

Borges C, Sherma N . Techniques for the analysis of cysteine sulfhydryls and oxidative protein folding. Antioxid Redox Signal. 2014; 21(3):511-31. PMC: 4076987. DOI: 10.1089/ars.2013.5559. View

Jang L, Keng H . Modified fabrication process of protein chips using a short-chain self-assembled monolayer. Biomed Microdevices. 2007; 10(2):203-11. DOI: 10.1007/s10544-007-9126-7. View

Kumar S, Aaron J, Sokolov K . Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties. Nat Protoc. 2008; 3(2):314-20. DOI: 10.1038/nprot.2008.1. View

Lijowski M, Caruthers S, Hu G, Zhang H, Scott M, Williams T . High sensitivity: high-resolution SPECT-CT/MR molecular imaging of angiogenesis in the Vx2 model. Invest Radiol. 2008; 44(1):15-22. PMC: 2703786. DOI: 10.1097/RLI.0b013e31818935eb. View

Shaw A, Hoffecker I, Smyrlaki I, Rosa J, Grevys A, Bratlie D . Binding to nanopatterned antigens is dominated by the spatial tolerance of antibodies. Nat Nanotechnol. 2019; 14(2):184-190. PMC: 6420075. DOI: 10.1038/s41565-018-0336-3. View

Gade T, Buchanan I, Motley M, Mazaheri Y, Spees W, Koutcher J . Imaging intratumoral convection: pressure-dependent enhancement in chemotherapeutic delivery to solid tumors. Clin Cancer Res. 2009; 15(1):247-55. PMC: 4217124. DOI: 10.1158/1078-0432.CCR-08-0611. View

Luke G, Yeager D, Emelianov S . Biomedical applications of photoacoustic imaging with exogenous contrast agents. Ann Biomed Eng. 2011; 40(2):422-37. DOI: 10.1007/s10439-011-0449-4. View

Veiseh O, Gunn J, Zhang M . Design and fabrication of magnetic nanoparticles for targeted drug delivery and imaging. Adv Drug Deliv Rev. 2009; 62(3):284-304. PMC: 2827645. DOI: 10.1016/j.addr.2009.11.002. View

10.

Jazayeri M, Amani H, Pourfatollah A, Avan A, Ferns G, Pazoki-Toroudi H . Enhanced detection sensitivity of prostate-specific antigen via PSA-conjugated gold nanoparticles based on localized surface plasmon resonance: GNP-coated anti-PSA/LSPR as a novel approach for the identification of prostate anomalies. Cancer Gene Ther. 2016; 23(10):365-369. DOI: 10.1038/cgt.2016.42. View

11.

Polo E, Puertas S, Moros M, Batalla P, Guisan J, de la Fuente J . Tips for the functionalization of nanoparticles with antibodies. Methods Mol Biol. 2013; 1051:149-63. DOI: 10.1007/978-1-62703-550-7_11. View

12.

Adler M, Dimitrov D . Therapeutic antibodies against cancer. Hematol Oncol Clin North Am. 2012; 26(3):447-81, vii. PMC: 3334873. DOI: 10.1016/j.hoc.2012.02.013. View

13.

Aydogan B, Li J, Rajh T, Chaudhary A, Chmura S, Pelizzari C . AuNP-DG: deoxyglucose-labeled gold nanoparticles as X-ray computed tomography contrast agents for cancer imaging. Mol Imaging Biol. 2010; 12(5):463-7. DOI: 10.1007/s11307-010-0299-8. View

14.

Mitri Z, Constantine T, ORegan R . The HER2 Receptor in Breast Cancer: Pathophysiology, Clinical Use, and New Advances in Therapy. Chemother Res Pract. 2013; 2012:743193. PMC: 3539433. DOI: 10.1155/2012/743193. View

15.

Chen G, Chen W, Wu Z, Yuan R, Li H, Gao J . MRI-visible polymeric vector bearing CD3 single chain antibody for gene delivery to T cells for immunosuppression. Biomaterials. 2009; 30(10):1962-70. DOI: 10.1016/j.biomaterials.2008.12.043. View

16.

Dvorak H . Leaky tumor vessels: consequences for tumor stroma generation and for solid tumor therapy. Prog Clin Biol Res. 1990; 354A:317-30. View

17.

Nath N, Chilkoti A . Label free colorimetric biosensing using nanoparticles. J Fluoresc. 2004; 14(4):377-89. DOI: 10.1023/b:jofl.0000031819.45448.dc. View

18.

Tallawi M, Rosellini E, Barbani N, Cascone M, Rai R, Saint-Pierre G . Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review. J R Soc Interface. 2015; 12(108):20150254. PMC: 4528590. DOI: 10.1098/rsif.2015.0254. View

19.

Sun I, Eun D, Na J, Lee S, Kim I, Youn I . Heparin-coated gold nanoparticles for liver-specific CT imaging. Chemistry. 2009; 15(48):13341-7. DOI: 10.1002/chem.200902344. View

20.

Ahmad Z, Yeap S, Ali A, Ho W, Mohamed Alitheen N, Hamid M . scFv antibody: principles and clinical application. Clin Dev Immunol. 2012; 2012:980250. PMC: 3312285. DOI: 10.1155/2012/980250. View