Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2021 Mar 15

PMID 33718375

Citations 8

Authors

Elena Morelli

Elisa A Speranza

Enrica Pellegrino

Galina V Beznoussenko

Francesca Carminati

Massimiliano Garre

Alexander A Mironov

Marco Onorati

Thomas Vaccari

Affiliations

Soon will be listed here.

Abstract

Snap29 is a conserved regulator of membrane fusion essential to complete autophagy and to support other cellular processes, including cell division. In humans, inactivating mutations causes CEDNIK syndrome, a rare multi-systemic disorder characterized by congenital neuro-cutaneous alterations. The fibroblasts of CEDNIK patients show alterations of the Golgi apparatus (GA). However, whether and how Snap29 acts at the GA is unclear. Here we investigate SNAP29 function at the GA and endoplasmic reticulum (ER). As part of the elongated structures in proximity to these membrane compartments, a pool of SNAP29 forms a complex with Syntaxin18, or with Syntaxin5, which we find is required to engage SEC22B-loaded vesicles. Consistent with this, in HeLa cells, in neuroepithelial stem cells, and , decreased SNAP29 activity alters GA architecture and reduces ER to GA trafficking. Our data reveal a new regulatory function of Snap29 in promoting secretory trafficking.

Citing Articles

Syntaxin-5's flexibility in SNARE pairing supports Golgi functions.

DSouza Z, Pokrovskaya I, Lupashin V Traffic. 2023; 24(8):355-379.

PMID: 37340984 PMC: 10330844. DOI: 10.1111/tra.12903.

The Regulated Secretion and Models of Intracellular Transport: The Goblet Cell as an Example.

Mironov A, Beznoussenko G Int J Mol Sci. 2023; 24(11).

PMID: 37298509 PMC: 10253439. DOI: 10.3390/ijms24119560.

siRNA screening reveals that SNAP29 contributes to exosome release.

Hessvik N, Sagini K, Romero S, Ramirez-Garrastacho M, Rodriguez M, Tutturen A Cell Mol Life Sci. 2023; 80(7):177.

PMID: 37285022 PMC: 10247572. DOI: 10.1007/s00018-023-04822-8.

Microcephaly-associated protein WDR62 shuttles from the Golgi apparatus to the spindle poles in human neural progenitors.

DellAmico C, Angulo Salavarria M, Takeo Y, Saotome I, Dellanno M, Galimberti M Elife. 2023; 12.

PMID: 37272619 PMC: 10241521. DOI: 10.7554/eLife.81716.

Syntaxin 18 regulates the DNA damage response and epithelial-to-mesenchymal transition to promote radiation resistance of lung cancer.

Thumser-Henner C, Oeck S, Kalmbach S, Forster J, Kindl F, Sak A Cell Death Dis. 2022; 13(6):529.

PMID: 35668077 PMC: 9170725. DOI: 10.1038/s41419-022-04978-4.

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