Downregulation of METTL7B Inhibits Proliferation of Human Clear Cell Renal Cancer Cells and

Overview

Journal Front Oncol

Specialty Oncology

Date 2021 Mar 15

PMID 33718220

Citations 12

Authors

Wei Li

Shi Xu

Naixiong Peng

Zejian Zhang

Hua He

Ruoyu Chen

Dong Chen

Jiqing Fan

Xisheng Wang

Affiliations

Soon will be listed here.

Abstract

Clear cell renal cell carcinoma (ccRCC) is the most aggressive urologic tumor, and its incidence and diagonosis have been continuously increasing. Identifying novel molecular biomarker for inhibiting the progression of ccRCC will facilitate developing new treatment strategies. Although methyltransferase-like 7B (METTL7B) was identified as a Golgi-associated methyltransferase, the function and mechanism of METTL7B in ccRCC development and progression has not been explored. METTL7B expression were significantly upregulated in ccRCC tissues (n = 60), which significantly associated with TNM classification, tumor size, lymph node metastasis, and poor prognosis for ccRCC patients. Functional studies showed downregulation of METTL7B inhibited cell proliferation, migration , and xenograft tumor formation . In addition, METTL7B knockdown promoted cell cycle arrest at G0/G1phase and induced cellular apoptosis. Taken together, downregulation of METTL7B inhibits ccRCC cell proliferation and tumorigenesis and . These findings provide a rationale for using METTL7B as a potential therapeutic target in ccRCC patients.

Citing Articles

EGR1 inhibits clear cell renal cell carcinoma proliferation and metastasis via the MAPK15 pathway.

Peng N, Cai Y, Chen D, Deng L, Zhang Z, Li W Oncol Res. 2025; 33(2):347-356.

PMID: 39866235 PMC: 11753989. DOI: 10.32604/or.2024.056039.

Potential prognostic and predictive biomarkers: METTL5, METTL7A, and METTL7B expression in gastrointestinal cancers.

Heydari S, Peymani M, Hashemi M, Ghaedi K, Entezari M Mol Biol Rep. 2025; 52(1):151.

PMID: 39847131 DOI: 10.1007/s11033-024-10207-2.

Methyltransferase-like 7B participates in bladder cancer via ACSL3 mA modification in a ferroptosis manner.

He J, Dong C, Song X, Qiu Z, Zhang H, Jiang Y Biol Direct. 2025; 20(1):9.

PMID: 39833962 PMC: 11744867. DOI: 10.1186/s13062-025-00597-z.

The role of RNA-modifying proteins in renal cell carcinoma.

Alhammadi M, Bajbouj K, Talaat I, Hamoudi R Cell Death Dis. 2024; 15(3):227.

PMID: 38503745 PMC: 10951318. DOI: 10.1038/s41419-024-06479-y.

Glycolysis Modulation by METTL7B Shapes Acute Lymphoblastic Leukemia Cell Proliferation and Chemotherapy Response.

Zhang L, Liu X, Zhou S, Wang P, Zhang X Hum Cell. 2024; 37(2):478-490.

PMID: 38294636 DOI: 10.1007/s13577-024-01025-6.

References

Zang W, Yang X, Wang T, Wang Y, Du Y, Chen X . MiR-451 inhibits proliferation of esophageal carcinoma cell line EC9706 by targeting CDKN2D and MAP3K1. World J Gastroenterol. 2015; 21(19):5867-76. PMC: 4438020. DOI: 10.3748/wjg.v21.i19.5867. View

Zhao Y, Tao Z, Chen X . Identification of a three-m6A related gene risk score model as a potential prognostic biomarker in clear cell renal cell carcinoma. PeerJ. 2020; 8:e8827. PMC: 7085294. DOI: 10.7717/peerj.8827. View

Zhang Y, Weinberg R . Epithelial-to-mesenchymal transition in cancer: complexity and opportunities. Front Med. 2018; 12(4):361-373. PMC: 6186394. DOI: 10.1007/s11684-018-0656-6. View

Chen W, Hill H, Christie A, Kim M, Holloman E, Pavia-Jimenez A . Targeting renal cell carcinoma with a HIF-2 antagonist. Nature. 2016; 539(7627):112-117. PMC: 5340502. DOI: 10.1038/nature19796. View

Bohnsack K, Hobartner C, Bohnsack M . Eukaryotic 5-methylcytosine (m⁵C) RNA Methyltransferases: Mechanisms, Cellular Functions, and Links to Disease. Genes (Basel). 2019; 10(2). PMC: 6409601. DOI: 10.3390/genes10020102. View

Bienvenu F, Jirawatnotai S, Elias J, Meyer C, Mizeracka K, Marson A . Transcriptional role of cyclin D1 in development revealed by a genetic-proteomic screen. Nature. 2010; 463(7279):374-8. PMC: 2943587. DOI: 10.1038/nature08684. View

Shimazu T, Barjau J, Sohtome Y, Sodeoka M, Shinkai Y . Selenium-based S-adenosylmethionine analog reveals the mammalian seven-beta-strand methyltransferase METTL10 to be an EF1A1 lysine methyltransferase. PLoS One. 2014; 9(8):e105394. PMC: 4140779. DOI: 10.1371/journal.pone.0105394. View

Li W, Yu K, Ma J, Shen J, Cheng C, Zhou F . Non-thermal plasma induces mitochondria-mediated apoptotic signaling pathway via ROS generation in HeLa cells. Arch Biochem Biophys. 2017; 633:68-77. DOI: 10.1016/j.abb.2017.09.005. View

Heyn H, Esteller M . An Adenine Code for DNA: A Second Life for N6-Methyladenine. Cell. 2015; 161(4):710-3. DOI: 10.1016/j.cell.2015.04.021. View

10.

Lamouille S, Xu J, Derynck R . Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol. 2014; 15(3):178-96. PMC: 4240281. DOI: 10.1038/nrm3758. View

11.

Yang X, Zhang S, He C, Xue P, Zhang L, He Z . METTL14 suppresses proliferation and metastasis of colorectal cancer by down-regulating oncogenic long non-coding RNA XIST. Mol Cancer. 2020; 19(1):46. PMC: 7047419. DOI: 10.1186/s12943-020-1146-4. View

12.

Rogawski D, Grembecka J, Cierpicki T . H3K36 methyltransferases as cancer drug targets: rationale and perspectives for inhibitor development. Future Med Chem. 2016; 8(13):1589-607. PMC: 5020427. DOI: 10.4155/fmc-2016-0071. View

13.

Ye D, Jiang Y, Sun Y, Li Y, Cai Y, Wang Q . METTL7B promotes migration and invasion in thyroid cancer through epithelial-mesenchymal transition. J Mol Endocrinol. 2019; 63(1):51-61. DOI: 10.1530/JME-18-0261. View

14.

Mende N, Kuchen E, Lesche M, Grinenko T, Kokkaliaris K, Hanenberg H . CCND1-CDK4-mediated cell cycle progression provides a competitive advantage for human hematopoietic stem cells in vivo. J Exp Med. 2015; 212(8):1171-83. PMC: 4516798. DOI: 10.1084/jem.20150308. View

15.

Peng W, Li J, Chen R, Gu Q, Yang P, Qian W . Upregulated METTL3 promotes metastasis of colorectal Cancer via miR-1246/SPRED2/MAPK signaling pathway. J Exp Clin Cancer Res. 2019; 38(1):393. PMC: 6729001. DOI: 10.1186/s13046-019-1408-4. View

16.

Xiong Y, Yuan L, Chen L, Zhu Y, Zhang S, Liu X . Identifying a Novel Biomarker of Clear Cell Renal Cell Carcinoma (ccRCC) Associated with Smoking by Co-Expression Network Analysis. J Cancer. 2018; 9(21):3912-3922. PMC: 6218786. DOI: 10.7150/jca.25900. View

17.

Wu C, MacCoss M, Mardones G, Finnigan C, Mogelsvang S, Yates 3rd J . Organellar proteomics reveals Golgi arginine dimethylation. Mol Biol Cell. 2004; 15(6):2907-19. PMC: 420113. DOI: 10.1091/mbc.e04-02-0101. View

18.

Barth D, Slaby O, Klec C, Juracek J, Drula R, Calin G . Current Concepts of Non-Coding RNAs in the Pathogenesis of Non-Clear Cell Renal Cell Carcinoma. Cancers (Basel). 2019; 11(10). PMC: 6826455. DOI: 10.3390/cancers11101580. View

19.

Xing T, He H . Epigenomics of clear cell renal cell carcinoma: mechanisms and potential use in molecular pathology. Chin J Cancer Res. 2016; 28(1):80-91. PMC: 4779762. DOI: 10.3978/j.issn.1000-9604.2016.02.09. View

20.

Horning B, Suciu R, Ghadiri D, Ulanovskaya O, Matthews M, Lum K . Chemical Proteomic Profiling of Human Methyltransferases. J Am Chem Soc. 2016; 138(40):13335-13343. PMC: 5072128. DOI: 10.1021/jacs.6b07830. View