Function of the Tof Gene Product in Modifying Chemical Stability of Trp Messenger RNA Synthesized from the PL Promoter of Lambda Trp Phage

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1977 Oct 20

PMID 337123

Citations 3

Authors

T Yamamoto

F Imamoto

Affiliations

Soon will be listed here.

Abstract

The trp operon translocated into the early region of phage lambda can be transcribed under the control of two promoters, the authentic trp promoter (Ptrptrp mRNA) and the PL promoter of the N gene (PLtrp mRNA) (Imamoto and Tani, 1972; Ihara and Imamoto, 1976a). PLtrp mRNA is stabilized with time after infection: at early times after infection chemical degradation of PLtrp mRNA is two-fold slower than for Ptrptrp mRNA, while at later times the stabilization of PLtrp mRNA is almost total. The stabilization of PLtrp mRNA is markedly reduced when the activity of the tof gene product is low due to a missense mutation of the tof gene. In contrast there is no significant reduction in stabilization when N function is lost by an amber mutation. On the basis of these and other experiments with lambdatrp susN7 tof12 phage, it is inferred that stabilization of the PLtrp mRNA is brought about by a modification of the "decay trigger", at least in part by the protein product of the tof gene.

Citing Articles

Cloning, sequence analysis, and expression of alteration of the mRNA stability gene (ams+) of Escherichia coli.

Chanda P, Ono M, Kuwano M, Kung H J Bacteriol. 1985; 161(1):446-9.

PMID: 2578448 PMC: 214894. DOI: 10.1128/jb.161.1.446-449.1985.

PL-promoted transcription of the promoter-proximal N-trp region is insensitive to chloramphenicol in the absence of N function.

Nakamura H, Yamamoto T, Imamoto F Mol Gen Genet. 1978; 159(1):21-6.

PMID: 634276 DOI: 10.1007/BF00401743.

Decay rates of Escherichia coli trp messenger RNA molecules lacking the normal 5'-terminal sequences.

Kano Y, Nakamura H, SOMERVILLE R, Imamoto F Mol Gen Genet. 1979; 176(3):379-84.

PMID: 392239 DOI: 10.1007/BF00333101.

References

Takeda Y, Ogata K, Matsubara K . Genetic analysis of the tof gene in the bacteriophage lambda genome. Virology. 1975; 65(2):385-91. DOI: 10.1016/0042-6822(75)90044-6. View

Levy S . Very stable prokaryotic messenger RNA in chromosomeless Escherichia coli minicells. Proc Natl Acad Sci U S A. 1975; 72(8):2900-4. PMC: 432886. DOI: 10.1073/pnas.72.8.2900. View

Jaenisch R, Jacob E, Hofschneider P . Replication of the small coliphage M13: evidence for long-living M13 specific messenger RNA. Nature. 1970; 227(5253):59-60. DOI: 10.1038/227059a0. View

Fiandt M, SZYBALSKI W . Physical mapping of the trp endpoint in the n-tL segment of phage lambda trpE-A. Virology. 1974; 61(1):312-4. DOI: 10.1016/0042-6822(74)90268-2. View

Imamoto F . Intragenic initiations of transcription of the tryptophan operon in Escherichia coli following dinitrophenol treatment without tryptophan. J Mol Biol. 1969; 43(1):51-69. DOI: 10.1016/0022-2836(69)90078-3. View

Hirashima A, Childs G, Inouye M . Differential inhibitory effects of antibiotics on the biosynthesis of envelope proteins of Escherichia coli. J Mol Biol. 1973; 79(2):373-89. DOI: 10.1016/0022-2836(73)90012-0. View

Kaiser A, Hogness D . The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg. J Mol Biol. 1960; 2:392-415. DOI: 10.1016/s0022-2836(60)80050-2. View

Yamamoto T, Imamoto F . Differential stability of trp messenger RNA synthesized originating at the trp promoter and pL promoter of lambda trp phage. J Mol Biol. 1975; 92(2):289-304. DOI: 10.1016/0022-2836(75)90228-4. View

Vogel H, Bonner D . Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956; 218(1):97-106. View

10.

Summers W . The process of infection with coliphage T7. IV. Stability of RNA in bacteriophage-infected cells. J Mol Biol. 1970; 51(3):671-8. DOI: 10.1016/0022-2836(70)90015-x. View

11.

Ihara S, Imamoto F . Differential sensitivity to antibiotics of trp mRNA synthesis originating at the trp promoter and the lambda promoter. Biochim Biophys Acta. 1976; 432(2):212-22. DOI: 10.1016/0005-2787(76)90163-5. View

12.

Lee N, Inouye M . Outer membrane proteins of Escherichia coli: biosynthesis and assembly. FEBS Lett. 1974; 39(2):167-70. DOI: 10.1016/0014-5793(74)80043-8. View

13.

Hattman S, Hofschneider P . Interference of bacteriophage T4 in the reproduction of RNA-phage M12. J Mol Biol. 1967; 29(1):173-90. DOI: 10.1016/0022-2836(67)90189-1. View

14.

Gupta R, Schlessinger D . Differential modes of chemical decay for early and late lambda messenger RNA. J Mol Biol. 1975; 92(2):311-8. DOI: 10.1016/0022-2836(75)90230-2. View

15.

Imamoto F, Tani S . Diversity of regulation of genetic transcription. Nat New Biol. 1972; 240(101):172-5. DOI: 10.1038/newbio240172a0. View

16.

Imamoto F, Schlessinger D . Bearing of some recent results on the mechanisms of polarity and messenger RNA stability. Mol Gen Genet. 1974; 135(1):29-38. DOI: 10.1007/BF00433898. View

17.

Nishimune Y . Prophage excision: specific gene amplification of excision-impaired lysogen after induction. Virology. 1973; 53(1):236-46. DOI: 10.1016/0042-6822(73)90482-0. View

18.

Pero J . Location of the phage lambda gene responsible for turning off lambda-exonuclease synthesis. Virology. 1970; 40(1):65-71. DOI: 10.1016/0042-6822(70)90379-x. View

19.

Takeda Y, Kuwano M . Synthesis and degradation of early mRNA in lambda phage. Biochim Biophys Acta. 1975; 407(1):83-90. DOI: 10.1016/0005-2787(75)90025-8. View

20.

Sato K, Nishimune Y, Sato M, Numich R, Matsushiro A, Inokuchi H . Suppressor-sensitive mutants of coliphage phi80. Virology. 1968; 34(4):637-49. View