DNA-Directed Protein Packing Within Single Crystals

Overview

Journal Chem

Publisher Elsevier

Date 2021 Mar 12

PMID 33709040

Citations 9

Authors

Peter H Winegar

Oliver G Hayes

Janet R McMillan

C Adrian Figg

Pamela J Focia

Chad A Mirkin

Affiliations

Soon will be listed here.

Abstract

Designed DNA-DNA interactions are investigated for their ability to modulate protein packing within single crystals of mutant green fluorescent proteins (mGFPs) functionalized with a single DNA strand (mGFP-DNA). We probe the effects of DNA sequence, length, and protein-attachment position on the formation and protein packing of mGFP-DNA crystals. Notably, when complementary mGFP-DNA conjugates are introduced to one another, crystals form with nearly identical packing parameters, regardless of sequence if the number of bases is equivalent. DNA complementarity is essential, because experiments with non-complementary sequences produce crystals with different protein arrangements. Importantly, the DNA length and its position of attachment on the protein markedly influence the formation of and protein packing within single crystals. This work shows how designed DNA interactions can be used to influence the growth and packing in X-ray diffraction quality protein single crystals and is thus an important step forward in protein crystal engineering.

Citing Articles

DNA-Regulated Multi-Protein Complement Control.

Ma Y, Winegar P, Figg C, Ramani N, Anderson A, Ngo K J Am Chem Soc. 2024; 146(48):32912-32918.

PMID: 39569872 PMC: 11755408. DOI: 10.1021/jacs.4c11315.

An Ensemble Classifiers for Improved Prediction of Native-Non-Native Protein-Protein Interaction.

Pratiwi N, Tayara H, Chong K Int J Mol Sci. 2024; 25(11).

PMID: 38892144 PMC: 11172808. DOI: 10.3390/ijms25115957.

DNA-Nanostructure-Guided Assembly of Proteins into Programmable Shapes.

Lu Q, Xu Y, Poppleton E, Zhou K, Sulc P, Stephanopoulos N Nano Lett. 2024; 24(5):1703-1709.

PMID: 38278134 PMC: 10853956. DOI: 10.1021/acs.nanolett.3c04497.

Modular Protein-DNA Cocrystals as Precise, Programmable Assembly Scaffolds.

Orun A, Shields E, Dmytriw S, Vajapayajula A, Slaughter C, Snow C ACS Nano. 2023; 17(14):13110-13120.

PMID: 37407546 PMC: 10373652. DOI: 10.1021/acsnano.2c07282.

Modular Nucleic Acid Scaffolds for Synthesizing Monodisperse and Sequence-Encoded Antibody Oligomers.

Winegar P, Figg C, Teplensky M, Ramani N, Mirkin C Chem. 2022; 8(11):3018-3030.

PMID: 36405374 PMC: 9674055. DOI: 10.1016/j.chempr.2022.07.003.

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