Phylogenetics and Pathology of Hydatid Disease in Slaughtered Buffaloes of North India

Overview

Journal Acta Parasitol

Specialty Parasitology

Date 2021 Mar 9

PMID 33687645

Citations 7

Authors

Aman D Moudgil

Anil K Nehra

Vikas Nehra

Rakshita Sharma

Sukhdeep Vohra

Pallavi Moudgil

Affiliations

Soon will be listed here.

Abstract

Purpose: Hydatid disease is one of the neglected and challenging (for diagnosis as well as for treatment) parasitic diseases. Along with adverse effect on animal's health leading to production losses, hydatidosis is also associated with huge economic losses. The present study was envisaged with an aim to assess the phylogeny and pathological changes due to natural hydatid cysts in lungs and liver of slaughtered buffaloes in north India.

Methods: A total of 137 slaughtered buffaloes intended for human consumption were screened for the presence of cysts. The retrieved cysts were confirmed molecularly based on the amplification of mitochondrial cytochrome oxidase 1 gene (mtCO1), exhibiting a product size of approximately 446 bp. The samples collected from infected lungs and liver were subjected to histopathological examination.

Results: The hydatid cysts were recorded in 25 (18.2%) animals. Phylogenetic analysis revealed the isolated strain to be closely related to Echinococcus granulosus sensu stricto (G1) genotype. The nucleotide diversity (π) obtained was 0.014685, whereas, Tajima's D was negative (- 2.796053), which indicated purifying selection or recent population expansion. Histopathologically, in the infected lungs, fibrosis and inflammatory reaction comprising of mononuclear cells and fibroblasts around the thick coat of granulation tissue were observed. Marked calcified masses and necrosis were also observed in the calcified cysts. However, in case of infected livers, Kupffer cell hyperplasia, degeneration of hepatocytes, fibrosis and inflammatory cells were most commonly observed around the hydatid cysts.

Conclusion: The findings of the present study are of significant veterinary and medical importance owing to economic and public health impact of G1 genotype of E. granulosus.

Citing Articles

In vitro and ex vivo protoscolicidal effect of poly(amidoamine) nanoemulsion against Echinococcus granulosus.

Aboelsoued D, Toaleb N, Ibrahim S, Ibrahim S Sci Rep. 2024; 14(1):11957.

PMID: 38796499 PMC: 11127951. DOI: 10.1038/s41598-024-62015-0.

Understanding the role of pigs in the transmission of zoonotic Echinococcus ortleppi in Haryana, India.

Moudgil A, Nehra A, Moudgil P Parasitol Res. 2024; 123(3):172.

PMID: 38536510 DOI: 10.1007/s00436-024-08192-3.

Cladistics of Echinococcus granulosus Sensu Stricto Genotypes Infecting the Slaughtered Pigs.

Moudgil A, Nehra A, Vohra S, Kumari A, Moudgil P Acta Parasitol. 2023; 68(4):754-761.

PMID: 37592063 DOI: 10.1007/s11686-023-00709-7.

First record of molecular and pathological investigations of physalopterid larvae infection in northern palm squirrel Funambulus pennantii.

Moudgil A, Nehra A, Vohra S, Nehra V, Lather D Parasitol Res. 2023; 122(7):1671-1675.

PMID: 37195506 DOI: 10.1007/s00436-023-07873-9.

The Monetary Losses Associated with Hydatidosis in Slaughtered Ruminants in Turkey.

Acioz M, Bozkaya F Helminthologia. 2023; 59(3):246-252.

PMID: 36694834 PMC: 9831519. DOI: 10.2478/helm-2022-0028.

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