Brucella Species Circulating in Rural and Periurban Dairy Cattle Farms: a Comparative Study in an Endemic Area

Overview

Journal Trop Anim Health Prod

Publisher Springer

Specialties Tropical Medicine
Veterinary Medicine

Date 2021 Mar 9

PMID 33686494

Citations 8

Authors

Saeed Alamian

Karim Amiry

Akram Bahreinipour

Afshar Etemadi

Majid Tebianian

Mohammad Hossein Fallah Mehrabadi

Maryam Dadar

Affiliations

Soon will be listed here.

Abstract

Brucellosis is among the most important zoonotic infectious diseases worldwide affecting both humans and domestic animals. The present study aimed to determine and compare the seroprevalence of brucellosis among rural and periurban dairy cattle farms of four Iranian provinces from 2017 to 2019. We applied different serological tests, including RBT, SAT, and iELISA to evaluate the brucellosis prevalence among 2808 dairy cattle. Species-specific multiplex PCR and biotyping tests were also used to further identify the implicated Brucella species. Serological screening using RBT, SAT, and iELISA led to 157 (5.6%), 112 (3.9%), and 139 (4.9%) positive results among tested cattle, respectively. Brucella abortus biovars 1 (2 cases) and biovars 3 (42 cases) were identified by biotyping experiments and multiplex PCR in all 44 tested lymph node samples. Further, Cohen's kappa statistical analysis revealed that the best degree of agreement was seen between RBT and iELISA (99.4%), followed by SAT/iELISA (98.5%) and finally RBT/SAT (98.4%). Our results also showed a significantly lower seroprevalence of brucellosis in periurban dairy cattle when compared to rural dairy cattle population (p value= 0.01). These results reflect the need for better vaccine coverage using RB51 combined with an appropriate test-and-slaughter program in the rural dairy cattle population.

Citing Articles

Bovine Brucellosis: First Comprehensive Evaluation from Hamedan, an Endemic Area in Iran.

Adabi M, Gharekhani J, Alamian S, Varasteh-Shams M, Fathi-Sheikhi M, Ghaderi H Indian J Microbiol. 2024; 64(4):1518-1527.

PMID: 39678977 PMC: 11645322. DOI: 10.1007/s12088-023-01152-y.

Detection of infection in raw milk of livestock in Famenin, West part of Iran.

Adabi M, Gharekhani J, Alamian S, Varasteh Shams M, Fathi Sheikh M, Shivapour Z Iran J Vet Res. 2024; 25(2):156-160.

PMID: 39624198 PMC: 11608534. DOI: 10.22099/IJVR.2024.49557.7292.

Characterization of spp. circulating in industrial dairy cattle farms in Iran: a field study 2016 - 2023.

Alamian S, Amiry K, Etemadi A, Dadar M Vet Res Forum. 2024; 15(4):195-202.

PMID: 38770201 PMC: 11102799. DOI: 10.30466/vrf.2024.2012972.4028.

Molecular Investigation of Species Belongs to Sheep and Goats in Seropositive Samples from an Endemic Area of Hamedan Province; Famenin Brucellosis Cohort Study.

Adabi M, Alamian S, Varasteh-Shams M, Ghaderi H, Shahbazi F, Gharekhani J Arch Razi Inst. 2024; 78(4):1349-1357.

PMID: 38226385 PMC: 10787933. DOI: 10.32592/ARI.2023.78.4.1349.

Shedding rate of Brucella spp. in the milk of seropositive and seronegative dairy cattle.

Abnaroodheleh F, Emadi A, Dashtipour S, Jamil T, Khaneghah A, Dadar M Heliyon. 2023; 9(4):e15085.

PMID: 37123977 PMC: 10133664. DOI: 10.1016/j.heliyon.2023.e15085.

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