The 28-kDa Calbindin-D is a Major Calcium-binding Protein in the Basilar Papilla of the Chick

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 May 1

PMID 3368450

Citations 21

Authors

J C Oberholtzer

C Buettger

M C Summers

F M Matschinsky

Affiliations

Soon will be listed here.

Abstract

In previous work we identified a basilar papilla protein (BPP23) that appears to be one of the most abundant soluble proteins in the basilar papilla of the chick cochlea. Here we report the purification of protein BPP23 from chick cochlea and the generation of a specific antiserum. Immunoblotting and immunoprecipitation experiments with this antiserum indicate that BPP23 is a calcium-binding protein very similar, if not identical, to avian calbindin, the 28-kDa vitamin D-dependent calcium-binding protein. Although the basilar papilla contains both receptor hair cells and supporting cells, immunocytochemical studies by others have localized calbindin-like immunoreactivity to the hair cells in the rat auditory receptor epithelium. Our estimates of the abundance of protein BPP23, assuming exclusive localization within the hair cell, indicate a concentration of at least 1 mM. Avian calbindin has four high-affinity (Kd = 0.5 X 10(-6)) calcium-binding sites. The presence of a specific calcium-binding activity at such high levels suggests an important function for cochlear calbindin (BPP23) in hair cell calcium homeostasis and auditory transduction.

Citing Articles

A Model for Link Pruning to Establish Correctly Polarized and Oriented Tip Links in Hair Bundles.

Tompkins N, Spinelli K, Choi D, Barr-Gillespie P Biophys J. 2017; 113(8):1868-1881.

PMID: 29045880 PMC: 5647544. DOI: 10.1016/j.bpj.2017.08.029.

The physiology of mechanoelectrical transduction channels in hearing.

Fettiplace R, Kim K Physiol Rev. 2014; 94(3):951-86.

PMID: 24987009 PMC: 4101631. DOI: 10.1152/physrev.00038.2013.

Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.

Avenarius M, Saylor K, Lundeberg M, Wilmarth P, Shin J, Spinelli K Mol Cell Proteomics. 2013; 13(2):606-20.

PMID: 24319057 PMC: 3916657. DOI: 10.1074/mcp.M113.033704.

Electrical tuning and transduction in short hair cells of the chicken auditory papilla.

Tan X, Beurg M, Hackney C, Mahendrasingam S, Fettiplace R J Neurophysiol. 2013; 109(8):2007-20.

PMID: 23365177 PMC: 3628031. DOI: 10.1152/jn.01028.2012.

Distinct energy metabolism of auditory and vestibular sensory epithelia revealed by quantitative mass spectrometry using MS2 intensity.

Spinelli K, Klimek J, Wilmarth P, Shin J, Choi D, David L Proc Natl Acad Sci U S A. 2012; 109(5):E268-77.

PMID: 22307652 PMC: 3277109. DOI: 10.1073/pnas.1115866109.

References

Celio M, Scharer L, Morrison J, Norman A, Bloom F . Calbindin immunoreactivity alternates with cytochrome c-oxidase-rich zones in some layers of the primate visual cortex. Nature. 1986; 323(6090):715-7. DOI: 10.1038/323715a0. View

Takahashi T, Carr C, Brecha N, Konishi M . Calcium binding protein-like immunoreactivity labels the terminal field of nucleus laminaris of the barn owl. J Neurosci. 1987; 7(6):1843-56. PMC: 6568887. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Crawford A, Fettiplace R . An electrical tuning mechanism in turtle cochlear hair cells. J Physiol. 1981; 312:377-412. PMC: 1275559. DOI: 10.1113/jphysiol.1981.sp013634. View

BREDDERMAN P, WASSERMAN R . Chemical composition, affinity for calcium, and some related properties of the vitamin D dependent calcium-binding protein. Biochemistry. 1974; 13(8):1687-94. DOI: 10.1021/bi00705a021. View

JANDE S, Maler L, LAWSON D . Immunohistochemical mapping of vitamin D-dependent calcium-binding protein in brain. Nature. 1981; 294(5843):765-7. DOI: 10.1038/294765a0. View

Hudspeth A . The cellular basis of hearing: the biophysics of hair cells. Science. 1985; 230(4727):745-52. DOI: 10.1126/science.2414845. View

Roth J, Bonner-Weir S, Norman A, Orci L . Immunocytochemistry of vitamin D-dependent calcium binding protein in chick pancreas: exclusive localization. Endocrinology. 1982; 110(6):2216-8. DOI: 10.1210/endo-110-6-2216. View

Kretsinger R . Structure and evolution of calcium-modulated proteins. CRC Crit Rev Biochem. 1980; 8(2):119-74. DOI: 10.3109/10409238009105467. View

10.

WASSERMAN R, Corradino R, Taylor A . Vitamin D-dependent calcium-binding protein. Purification and some properties. J Biol Chem. 1968; 243(14):3978-86. View

11.

Roth J, Baetens D, Norman A, Garcia-Segura L . Specific neurons in chick central nervous system stain with an antibody against chick intestinal vitamin D-dependent calcium-binding protein. Brain Res. 1981; 222(2):452-7. DOI: 10.1016/0006-8993(81)91054-4. View

12.

Christakos S, Rhoten W, Feldman S . Rat calbindin D28K: purification, quantitation, immunocytochemical localization, and comparative aspects. Methods Enzymol. 1987; 139:534-51. DOI: 10.1016/0076-6879(87)39111-6. View

13.

Vaitukaitis J . Production of antisera with small doses of immunogen: multiple intradermal injections. Methods Enzymol. 1981; 73(Pt B):46-52. DOI: 10.1016/0076-6879(81)73055-6. View

14.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

15.

JANDE S, Tolnai S, LAWSON D . Immunohistochemical localization of vitamin D-dependent calcium-binding protein in duodenum, kidney, uterus and cerebellum of chickens. Histochemistry. 1981; 71(1):99-116. DOI: 10.1007/BF00592574. View

16.

Baimbridge K, Miller J, Parkes C . Calcium-binding protein distribution in the rat brain. Brain Res. 1982; 239(2):519-25. DOI: 10.1016/0006-8993(82)90526-1. View

17.

Lewis R, Hudspeth A . Voltage- and ion-dependent conductances in solitary vertebrate hair cells. Nature. 1983; 304(5926):538-41. DOI: 10.1038/304538a0. View

18.

WASSERMAN R, Taylor A . Vitamin d3-induced calcium-binding protein in chick intestinal mucosa. Science. 1966; 152(3723):791-3. DOI: 10.1126/science.152.3723.791. View

19.

Higgins R, Dahmus M . Rapid visualization of protein bands in preparative SDS-polyacrylamide gels. Anal Biochem. 1979; 93(2):257-60. DOI: 10.1016/s0003-2697(79)80148-7. View

20.

Cohen G, Fermin C . The development of hair cells in the embryonic chick's basilar papilla. Acta Otolaryngol. 1978; 86(5-6):342-58. DOI: 10.3109/00016487809107513. View