Receptors for Phorbol Esters Are Primarily Localized in Neurons: Comparison of Neuronal and Glial Cultures

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1988 Jan 1

PMID 3368029

Citations 5

Authors

M K Raizada

C A Morse

R A Gonzales

F T Crews

C Sumners

Affiliations

Soon will be listed here.

Abstract

Binding of [3H]PDB has been measured in the present study to determine the levels of protein kinase C in the neuronal and astrocytic glial cells in culture from rat brain. Binding of [3H]PDB to homogenates of cultured neuronal cells from the brains of normotensive and hypertensive rats was time-dependent and specific. The relative potency for competition by various phorbol esters to [3H]PDB binding was TPA greater than beta-PDD greater than POE greater than alpha-PDD greater than or equal to 4 alpha phorbol. Scatchard analysis showed that neuronal cultures from normotensive rat brains contained 2-3 fold more phorbol ester receptors compared with the glial cultures from the same brains. No differences in the Kd and Bmax were observed between neuronal cultures from normotensive and spontaneously hypertensive rat brains. These studies suggest that the phorbol ester receptors are primarily localized in neuronal cells.

Citing Articles

The effect of acute and chronic electroconvulsive shock on [3H]phorbol-dibutyrate binding to rat brain membranes.

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Effects of phorbol ester on immunoreactive protein kinase C, insulin binding, and glucose uptake in astrocytic glial and neuronal cells from the brain.

Mudd L, Raizada M Neurochem Res. 1990; 15(3):273-8.

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Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C.

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Neurotrophic activity of platelet-derived growth factor (PDGF): Rat neuronal cells possess functional PDGF beta-type receptors and respond to PDGF.

Smits A, Kato M, Westermark B, Nister M, Heldin C, Funa K Proc Natl Acad Sci U S A. 1991; 88(18):8159-63.

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