Compounds Inhibit Oxytocin-Induced Signaling Pathways in Human Myometrial Cells

Overview

Journal Front Pharmacol

Date 2021 Mar 8

PMID 33679416

Citations 7

Authors

Stefanie Santos

Leonie Zurfluh

Monica Mennet

Olivier Potterat

Ursula von Mandach

Matthias Hamburger

Ana Paula Simoes-Wust

Affiliations

Soon will be listed here.

Abstract

has been used in the treatment of premature labor, first in anthroposophic hospitals and, recently, in conventional settings as an add-on medication. work with hTERT human myometrial cells showed that leaf press juice inhibits the increase of intracellular free calcium concentration induced by oxytocin, a hormone known to play a role in labor. Our aim was to identify fractions/compounds in press juice that contribute to this inhibitory effect, and to investigate their effect on oxytocin-driven activation of the MAPK cascade. Several fractions/compounds from press juice led to a concentration-dependent decrease of oxytocin-induced increase of intracellular free calcium concentration, but none of them was as strong as press juice. However, the combination of a bufadienolide and a flavonoid-enriched fraction was as effective as press juice, and their combination had a synergistic effect. press juice inhibited oxytocin-driven activation of MAPKs SAPK/JNK and ERK1/2, an effect also exerted by the bufadienolide-enriched fraction. The effect of press juice on oxytocin-induced signaling pathways was comparable to that of the oxytocin-receptor antagonist and tocolytic agent atosiban. Our findings further substantiate the use of press juice preparations in the treatment of preterm labor.

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