High-generation Near-isogenic Lines Combined with Multi-omics to Study the Mechanism of Polima Cytoplasmic Male Sterility

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2021 Mar 6

PMID 33673810

Citations 8

Authors

Benqi Wang

Zunaira Farooq

Lei Chu

Jie Liu

Huadong Wang

Jian Guo

Jinxing Tu

Chaozhi Ma

Cheng Dai

Jin Wen

Jinxiong Shen

Tingdong Fu

Bin Yi

Affiliations

Soon will be listed here.

Abstract

Background: Cytoplasmic male sterility (CMS), which naturally exists in higher plants, is a useful mechanism for analyzing nuclear and mitochondrial genome functions and identifying the role of mitochondrial genes in the plant growth and development. Polima (pol) CMS is the most universally valued male sterility type in oil-seed rape. Previous studies have described the pol CMS restorer gene Rfp and the sterility-inducing gene orf224 in oil-seed rape, located in mitochondria. However, the mechanism of fertility restoration and infertility remains unknown. Moreover, it is still unknown how the fecundity restorer gene interferes with the sterility gene, provokes the sterility gene to lose its function, and leads to fertility restoration.

Result: In this study, we used multi-omics joint analysis to discover candidate genes that interact with the sterility gene orf224 and the restorer gene Rfp of pol CMS to provide theoretical support for the occurrence and restoration mechanisms of sterility. Via multi-omics analysis, we screened 24 differential genes encoding proteins related to RNA editing, respiratory electron transport chain, anther development, energy transport, tapetum development, and oxidative phosphorylation. Using a yeast two-hybrid assay, we obtained a total of seven Rfp interaction proteins, with orf224 protein covering five interaction proteins.

Conclusions: We propose that Rfp and its interacting protein cleave the transcript of atp6/orf224, causing the infertility gene to lose its function and restore fertility. When Rfp is not cleaved, orf224 poisons the tapetum cells and anther development-related proteins, resulting in pol CMS mitochondrial dysfunction and male infertility. The data from the joint analysis of multiple omics provided information on pol CMS's potential molecular mechanism and will help breed B. napus hybrids.

Citing Articles

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Spatiotemporal profiles of gene activity in stamen delineate nucleo-cytoplasmic interaction in a male-sterile somatic cybrid citrus.

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A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in .

Zhang Y, An R, Song M, Xie C, Wei S, Wang D Plants (Basel). 2023; 12(7).

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Complete mitochondrial genome sequencing and identification of candidate genes responsible for C5-type cytoplasmic male sterility in cabbage ( var. ).

Zhong X, Yue X, Cui J, Han R, Gao Y, Kang J Front Plant Sci. 2022; 13:1019513.

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